茶叶科学2019，Vol.39Issue(1)：43-54,12.
L-茶氨酸对小鼠肠道形态结构及游离氨基酸的影响
Effects of L-theanine on Intestinal Morphology and Free Amino Acids in Mice
摘要
Abstract
Fifty KM male mice were randomly divided into the normal, glutamine and low, medium and high dose L-theanine treatment groups with 10 mice/group. The mice were continuously fed for 28 days to investigate the effects of L-theanine on intestinal morphology and free amino acids. The results show that compared with the normal group, the intestinal villus height increased with the increase of L-theanine dosage. The duodenal villus height in each L-theanine groups, the jejunum villus height and villus height/crypt depth in the middle and high dose L-theanine groups were significantly increased (P<0.05). The levels of free amino acids in serum, urea nitrogen in high- and middle-dose L-theanine groups were significantly decreased (P<0.05). Alkaline phosphatase and total protein were significantly increased in high-dose L-theanine group (P<0.05). The dose-dependent increase effect of L-theanine on the contents of free amino acids in different intestinal tract were also observed. In the duodenum, all essential amino acids and most of the non-essential amino acids in the L-theanine dose groups increased significantly (P<0.05). The essential amino acid contents in the high dose group were significantly higher than those in the low dose group (P<0.05). In the jejunum, the essential amino Thr, Lys and Ile in the low dose group and all essential amino ac ids in the middle and high dose groups were significantly increased. Other amino acids except Cit in the middle and high dose groups were significantly higher than those in the low dose group (P<0.05). In the ileum, all essential amino acids in the middle and high dose groups were significantly reduced, and other amino acid contents in the middle and high dose groups were significantly higher than the low dose group except for GABA ( P<0.05). The results suggest that L-theanine can improve the intestinal morphology of mice by increasing the villus height of the intestine and the villus height/crypt depth, promoting the absorption and utilization of amino acids in duodenum and jejunum.关键词
L-茶氨酸/营养干预/肠道形态结构/游离氨基酸/谷氨酰胺Key words
L-theanine； nutritional intervention； intestinal morphology； free amino acids； Glutamine分类
轻工纺织引用本文复制引用
彭影琦,肖文军,张盛,袁冬寅,林玲,周阳,龚志华..L-茶氨酸对小鼠肠道形态结构及游离氨基酸的影响[J].茶叶科学,2019,39(1):43-54,12.基金项目
国家自然科学基金(31871804)、国家重点研发计划(2017YFD0400803)、长沙市科技计划重大专项(kq1703003) （31871804）
