食品科学技术学报2019，Vol.37Issue(2)：28-35,8.DOI:10.3969/j.issn.2095-6002.2019.02.005
纳米球形聚电解质刷固定化酶合成及性质表征研究
Study on Synthesis and Characterization of Nano-Spherical Polyelectrolyte Brush Immobilized Enzyme
摘要
Abstract
The nano-spherical polyelectrolyte brush with polystyrene as the core and grafted acrylic mono-mer was synthesized by photoemulsion polymerization. The microsphere structure was characterized by transmission electron microscopy, Fourier transform infrared spectroscopy, nuclear magnetic resonance spectroscopy and laser particle size analyzer. The immobilization of alkaline protease was studied by using a ball brush as a carrier. The results showed that the prepared nano-spherical polyelectrolyte brush carrier had an average particle size of about 80 nm, uniform size, and good dispersion. The optimal immobiliza-tion conditions for alkaline protease were buffer pH 9. 0, immobilization time 6 h, and enzyme solution addition 1. 2 mg/mL. Compared with the free enzyme, the optimum pH ( changed from 11. 0 to 9. 0) . The optimum reaction temperature was 60℃, which increased 15℃ compared with free enzymes. Both temperature stability and acid-base stability were higher than free enzymes. The application range of the enzyme is widened, which is more conducive to industrial production.关键词
纳米球形聚电解质刷/碱性蛋白酶/固定化酶/游离酶/酶学性质Key words
nano-spherical polyelectrolyte brush/characterization/alkaline protease/immobilized enzyme/free enzyme/enzyme properties分类
轻工纺织引用本文复制引用
熊科,高思宇,柳佳芸,邓蕾,裴鹏钢,李秀婷..纳米球形聚电解质刷固定化酶合成及性质表征研究[J].食品科学技术学报,2019,37(2):28-35,8.基金项目
国家重点研发计划项目( 2017YFC1600605 ) （ 2017YFC1600605 ）
北京市自然科学基金资助项目( 6172003 ) （ 6172003 ）
国家自然科学基金资助项目(31601408). （31601408）
