食品科学技术学报2019，Vol.37Issue(2)：48-55,8.DOI:10.3969/j.issn.2095-6002.2019.02.008
不同单色光对紫色红曲霉生长、色素和桔霉素合成的影响
Effects of Different Monochromatic Light on Growth, Pigments and Citrinin Biosynthesis of Monascus purpureus
摘要
Abstract
Effects of different monochromatic lights ( red, yellow, green and blue) on culture morphology and six well known pigments production of Monascus purpureus M9 were investigated in this study. Fur-thermore, various red light intensities and illumination time on six well known pigments, citrinin produc-tion and expression levels of monascus pigments and citrinin biosynthetic gene cluster were examined using HPLC and RT-qPCR. The results showed that red light had the mostly significant effects on the growth and pigments production of Monascus purpureus M9. The optimal illumination time and illumina-tion intensity were 30 min/d and 300 lx. The expression levels of biosynthetic genes, such as mppA/B/D/F, mppR1/R2, MpPKS5, and MpFasA2/B2 were positively correlated with the production of orange pigments, and the expression levels of mppC and mppE were positively correlated with the yields of red and yellow pigments. Moreover, ctnA/D/E/F/G/H/I, orf1/3/4/5, pksCT were positively correlated with citrinin production and ctnR1 was negatively correlated with citrinin production.关键词
紫色红曲霉M9/单色光/生长/红曲色素/桔霉素Key words
Monascus purpureus M9/monochromatic light/growth/monascus pigments/citrinin分类
轻工纺织引用本文复制引用
刘宏,陈迪,陈勉华,李贞景,王昌禄..不同单色光对紫色红曲霉生长、色素和桔霉素合成的影响[J].食品科学技术学报,2019,37(2):48-55,8.基金项目
国家自然科学基金资助项目(31330059 （）
31571820). （）
