食品科学2019，Vol.40Issue(6)：253-260,8.DOI:10.7506/spkx1002-6630-20180103-040
同时测定不同人参加工产品中20种人参皂苷的UPLC-PDA方法开发和验证
Development of a Validated UPLC Method for Simultaneous Analyses of 20 Ginsenosides in Various Processed Ginseng Products
摘要
Abstract
A cost effective,simple,precise and accurate ultra-high performance liquid chromatography (UPLC) method was established and validated for simultaneous determination of 20 ginsenosides in various processed ginseng products,including Rg1,Re,Rf,20(S)-Rg2,20(R)-Rg2,Rb1,Rc,Ra1,Rb2,Rb3,Rd,Rk3,F2,20(S)-Rg3,20(R)-Rg3,compound K (CK),Rg5,20(S)-Rh2,20(R)-Rh2 and protopanaxadiol (PPD).The developed method was carried out on a Waters ACQUITY UPLC BEH C18 column (2.1 mm × 50 mm,1.7 μn) using a photodiode array detector (PDA).Good chromatographic separation was performed using a mixture of acetonitrile (B) and water (A) as the mobile phase with gradient elution at a flow rate of 0.3 mL/min and column temperature of 30 ℃ within 31 min.The method was well validated with respect to linearity (R2＞ 0.998),precision (intra-day RSD ＜ 4.65％ and inter-day RSD ＜ 4.88％) and accuracy (recovery rate between 85.71％ and 108.50％),and the limit of detection (LOD) and limit of quantification (LOQ) were in the range of 0.81-3.10 μg/mL and 2.88-10.00 μg/mL,respectively.This method was rapid and reliable and was successfully used for the analysis of various processed ginseng products,including freshly stored ginseng,red ginseng and white ginseng and the results revealed significant variations in the ginsenoside levels.This method can be suitable for the analysis of active compounds and the quality control of fresh and processed ginseng.关键词
人参产品/人参皂苷/超高效液相色谱/同时测定/质量控制Key words
ginseng products/ginsenosides/ultra-high performance liquid chromatography/simultaneous determination/quality control分类
医药卫生引用本文复制引用
王艳红,吴晓民,朱艳萍,赵丹,李月茹..同时测定不同人参加工产品中20种人参皂苷的UPLC-PDA方法开发和验证[J].食品科学,2019,40(6):253-260,8.基金项目
国家农产品质量安全风险评估重大专项(GJFP201701002) （GJFP201701002）
吉林省科技发展计划项目(20170203007YY) （20170203007YY）
