抑制食管癌STC-1基因表达对食管癌细胞凋亡、IL-1β和TNF-α表达及JAK2/STAT3信号的影响研究OA北大核心CSCDCSTPCD
Effect of STC-1 gene expression was inhibited on cell apoptosis, IL-1β and TNF-α expression and JAK2/STAT3 signal in esophageal carcinoma
目的:观察抑制食管癌斯钙素-1(STC-1)基因表达对食管癌细胞凋亡、IL-1β和TNF-α表达及JAK2/STAT3信号的影响.方法:RT-PCR及Western blot检测STC-1在人食管鳞状细胞癌KYSE170、Eca109、TE1和TE10细胞相对于正常人食管鳞状上皮细胞Het-1A的表达;将合成的STC-1的siRNA序列及无干扰作用的si NRA序列转染Eca109细胞,分别标记为STC-1-siRNA组和NC组,并设定空白对照组…查看全部>>
Objective: To observe the effect of STC-1 gene expression was inhibited on the apoptosis, IL-1β and TNF-α expression and JAK2/STAT3 signal in esophageal cancer cells. Methods: Compared with normal human esophageal squamous epithelial cells Het-1 A, STC-1 expression was detected in human esophageal squamous cell carcinoma KYSE170, Eca109, TE1 and TE10 cells by RTPCR and Western blot; the siRNA sequence that the synthesized STC-1 and the si NRA sequence withou…查看全部>>
卜秀梅;王文刚;李辉;郑瑾
辽宁中医药大学, 沈阳 110032沈阳医学院附属中心医院, 沈阳 110024辽宁省肿瘤医院, 沈阳 110042中国医科大学附属医院, 沈阳 110001
医药卫生
食管癌STC-1基因凋亡免疫JAK2/STAT3信号通路
Esophageal cancerSTC-1 geneApoptosisImmunizationJAK2/STAT3 signaling pathway
《中国免疫学杂志》 2019 (2)
186-191,6
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