茶叶科学2019，Vol.39Issue(4)：440-446,7.
固相萃取-超高效液相色谱串联质谱测定茶产品中吡蚜酮
Determination of Pymetrozine in Tea Products by Solid Phase Extraction-Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry
摘要
Abstract
An analytical method for the determination of pymetrozine residues in tea products, including dry tea, matcha and instant tea powder, was established based on Cleanert PCX solid phase extraction-ultra high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The residual pymetrozine in dry tea, matcha and instant tea powder was extracted by a mixture of methanol and water, cleaned up and concentrated by a Cleanert PCX SPE column, and separated on an Acquity BEH C18 column. Pymetrozine level was detected by UPLC-MS/MS on multiple reaction monitoring (MRM) mode and quantified with external standard method. In the spiked range of 0.005-1.000 mg·kg-1, the average recovery of pymetrozine was 77.0%-95.1%, and the relative standard deviation (RSD, n=5) ranged from 1.8%-6.9%. The limit of quantitation (LOQ) was 0.005 to 0.010 mg·kg-1. The sensitivity, precision and recovery of the developed method meet the requirements for residual pesticides analysis in various tea products. The results of this study can provide an analytical method for the determination and risk assessment of pymetrozine in tea products.关键词
吡蚜酮/茶/农药残留/固相萃取法/高效液相色谱-串联质谱Key words
pymetrozine/tea/pesticide residue/solid phase extraction/UPLC-MS/MS分类
农业科技引用本文复制引用
余焕,罗逢健,周利,林琴,杨洁,孙荷芝,伍旭东,王新茹,张新忠,陈宗懋..固相萃取-超高效液相色谱串联质谱测定茶产品中吡蚜酮[J].茶叶科学,2019,39(4):440-446,7.基金项目
国家重点研发计划(2016YFD0200207)、浙江省自然科学基金(LY18C160005)、中国农业科学院创新工程(CAAS-ASTIP-TRICAAS) （2016YFD0200207）
