茶叶科学2019，Vol.39Issue(5)：576-586,11.
固相萃取-GC/LC-MS/MS测定茶叶中79种农药残留
Determination of 79 Pesticide Residues in Tea by Solid Phase Extraction with GC-MS/MS and LC-MS/MS
摘要
Abstract
In order to detect various pesticide residues in tea, a reliable, rapid and high throughput detection method was established: a solid phase extraction was developed for the determination of 79 pesticide residues in tea by gas chromatography-tandem mass spectrometry (GC-MS/MS) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). These samples were extracted by 20 mL acetonitrile. 10 mL extracts were cleaned by Carbon/NH2 column with 20 mL acetonitrile + toluene (3∶1) and then mixed with 5 mL acetonitrile, then these procdssed samples were detected by GC-MS/MS and LC-MS/MS, respectively. The results show that the concentrations of 79 pesticide residues had good linearity relationships in the range of 0.01-0.40 mg·L-1, with the correlation coefficients (R2) above 0.995, the recoveries of high, low and medium levels were ranged from 67.3%-130.8%, the relative standard deviations (RSDs) were less than 15%, the limits of quantification (LOQs) of 98.7 % pesticides were less than or equal to 0.01 mg·kg-1, multiple types of tea were detected, and the results were proved to be qualified. Consequently, the method can simultaneously detect 5 kinds of pesticides, including organophosphorus, organochlorine, pyrethroids, carbamates and organic heterocyclic pesticides, which would be useful to determine multi-pesticide residues in high numbers of tea samples.关键词
茶叶/GC-MS/MS/LC-MS/MS/农药残留Key words
tea/GC-MS/MS/LC-MS/MS/pesticide residues分类
农业科技引用本文复制引用
曾艳,郎红,杨巧慧,李霞雪,史朝婷..固相萃取-GC/LC-MS/MS测定茶叶中79种农药残留[J].茶叶科学,2019,39(5):576-586,11.基金项目
雅安市重点科技项目(2017yyjskf05) （2017yyjskf05）
