食品科学技术学报2019，Vol.37Issue(5)：64-71,8.DOI:10.3969/j.issn.2095-6002.2019.05.008
Streptomyces alboflavus抗真菌物质对串珠镰刀菌的抑制机理研究
Inhibition Mechanism of Streptomyces alboflavus Antifungal Substance Against Fusarium moniliforme
摘要
Abstract
The inhibition mechanism of antifungal substance generated by Streptomyces alboflavus against Fusarium moniliforme was investigated. The antifungal substance was qualitatively analyzed by pH paper and Doskochilova system chromatography. The destructive effects of antifungal substance on cell mem-brane and cell wall of Fusarium moniliforme were observed by fluorescence microscopy and scanning elec-tron microscopy. The antagonistic effects of main components of cell membrane and wall on antifungal substance were explored. Furthermore, the inhibitory effect of antifungal substance on ergosterol in cell membrane of Fusarium moniliforme was detected by HPLC. The antifungal substance was neutral and non-water-soluble type I antibiotics by polarity test and Doskochilova system paper chromatography. Opti-cal microscope was used to observe the inhibition of germination of Fusarium moniliforme spores, the my-celia were distortion, deformation and swelling when treated with the antifungal substance, and the fluo-rescence microscope revealed that the permeability of mycelial cell membrane was changed. Moreover, the results from scanning electron microscope showed that the surface of vesicular mycelia was uneven and fragments remained. The antagonism of antifungal substance effect on components of cell wall and cell membrane and the detection by HPLC showed that the synthesis of ergosterol in the cell membrane was affected by antifungal substance. The synthesis of ergosterol in the cell membrane of Fusarium monili-forme was affected by the antifungal substance produced by Streptomyces alboflavus. This study laid a the-oretical foundation on the identification of antifungal substance and the application of antagonistic bacteria.关键词
白黄链霉菌/串珠镰刀菌/抑菌物质/抑菌活性/抑菌机理Key words
Streptomyces alboflavus/Fusarium moniliforme/antifungal substance/antifunga activity/antifungal mechanism分类
轻工纺织引用本文复制引用
王志芳,李贞景,杨明冠,路来风,王昌禄,李政,张健飞,巩继贤..Streptomyces alboflavus抗真菌物质对串珠镰刀菌的抑制机理研究[J].食品科学技术学报,2019,37(5):64-71,8.基金项目
天津市科技计划重点项目(16YFZCNC00700) （16YFZCNC00700）
国家自然科学基金资助项目(31701668) （31701668）
天津市自然科学基金资助项目(17JCQNJC14300). （17JCQNJC14300）
