现代食品科技2019，Vol.35Issue(9)：38-48,11.DOI:10.13982/j.mfst.1673-9078.2019.9.004
决明子蛋白水解产物的抗氧化活性:热和胃肠稳定性,肽鉴定和计算机分析
Antioxidant Activity of Semen Cassiae Protein Hydrolysate: Thermal and Gastrointestinal Stability, Peptide Identification, and In Silico Analysis
蔡俊泰 1徐芷菌 2徐国强 1李仁杰 3阿都马南·法希拉 3黄怀祖4
作者信息
- 1. 拉曼大学理学院,马来西亚金宝31900
- 2. 拉曼大学生物多样性研究中心,马来西亚金宝31900
- 3. 中国医科大学营养系,台湾台中40402
- 4. 马来西亚理工大学生物科学系,马来西亚新山81310
- 折叠
摘要
Abstract
Semen cassiae (SC),seeds of Cassia obtusifolia,is used as treatments for various diseases in Traditional Chinese Medicine.This study evaluated the antioxidant activity and stability of SC hydrolysate fractions and identified peptides in the most active fraction.SC proteins were hydrolyzed by alcalase for 2-6 hours;＜ 3000 u peptide fractions were produced via membrane uitrafiltration.UT 2 h (fraction produced from 2-hour hydrolysate) exhibited the strongest ABTS+ scavenging (EC50 =229 μg/mL) and iron chelating (EC50 =89 μg/mL) activities.Its ABTS.+ scavenging activity was retained after thermal treatment and simulated gastrointestinal (GI) digestion,but iron chelating activity was fully retained only after GI digestion.Peptides were purified from UF 2 h by using solid phase extraction,gel filtration chromatography and high performance liquid chromatography analysis,guided by ABTS.+ scavenging assay.Four peptides were identified by mass spectrometric analysis:PMPVR (599.29 u),FETLPF (752.34 u),KMRDNL (775.37 u),and LDESKRF (893.50 u).In silico analysis predicted that before simulated GI digestion,all four peptides were non-toxic and non-allergenic.After GI digestion,peptide fragments released from the four peptides were predicted to be still non-toxic,whereas fragments of only PMPVR and KMRDNL were predicted to be non-allergenic.Taken together,UF 2 h and the peptides contained in it are promising antioxidants for the development of functional food and nutraceuticals.关键词
致敏性/抗氧化肽/决明/胃肠稳定性/热稳定性Key words
allergenicity/antioxidant peptide/Cassia obtusifolia/gastrointestinal stability/thermal stability引用本文复制引用
蔡俊泰,徐芷菌,徐国强,李仁杰,阿都马南·法希拉,黄怀祖..决明子蛋白水解产物的抗氧化活性:热和胃肠稳定性,肽鉴定和计算机分析[J].现代食品科技,2019,35(9):38-48,11.
