茶叶科学2019，Vol.39Issue(1)：105-114,10.
超高效液相色谱-串联质谱联法测定茶园用农药制剂中烷基酚聚氧乙烯醚含量
Determination of Alkylphenol Ethoxy lates in Agrochemicals for Tea Garden by UPLC-MS/MS
摘要
Abstract
This study established an ultra high performance liquid chromatography-tandem mass spectrometry method for the determination of alkylphenol polyoxyethylene (APEOs) in pesticide formulations. APEOs were separated on a Waters-BEH C18 column, measured by a multi-reaction monitoring mode (MRM) with an electrospray positive ion source (ESI+), and quantified by an external standard method. The matrix standard curve of APEOs (n=3-20) in aqueous solutions, water emulsion, suspension concentrate, emulsifiable concentrate,granules and wettable powder had a good linearity in the range of 0.047-1 503.6μg·kg-1with the correlation coefficient (r) between 0.996 1 and 0.999 9. The recovery of APEOs spiked in the level of 0.5-1 503.6μg·kg-1 ranged from 83.8%-120.4% with relative standard deviation (RSD) of 0.6%-13.7%, and the limits of quantity (LOQs) of 0.5~150.4μg·kg-1. Among the 51 pesticide formulations used in tea gardens, the percentage of Octylphenol ethoxylates (OPEOs) was less than 0.5%. The mass fraction of nonylphenol ethoxylates (NPEOs) was ND-32.1% and the detection rate was 90.2%, indicating the wide use of NPEOs in pesticides in tea gardens.关键词
农药助剂/农药制剂/茶叶/烷基酚聚氧乙烯醚/超高效液相色谱-串联质谱Key words
pesticide additives； pesticide formulations； tea； alkylphenol ethoxylates； ultra high performance liquid chromatography-tandem mass spectrometry分类
农业科学引用本文复制引用
林琴,周利,罗逢健,杨洁,王新茹,孙荷芝,杨梅,陈宗懋..超高效液相色谱-串联质谱联法测定茶园用农药制剂中烷基酚聚氧乙烯醚含量[J].茶叶科学,2019,39(1):105-114,10.基金项目
科技部科技基础专项(2013FY110100)、浙江省自然科学基金(Y18C160027)、国家茶叶产业技术体系(CARS－23) （2013FY110100）
