食品科学技术学报2019，Vol.37Issue(6)：46-52,7.DOI:10. 3969/j. issn. 2095﹣6002. 2019. 06. 007
宣威火腿中抗菌肽的分离、纯化及鉴定
Isolation, Purification and Identification of Antimicrobial Peptides in Xuanwei Ham
摘要
Abstract
In order to explore the antibacterial activity and compositions of bioactive peptides generated in dry﹣cured ham, Xuanwei ham, which was produced at Qujing area of Yunnan China, was selected as the material to extract the crude peptides by buffer solution method. The antibacterial activity was evaluated based on growth inhibition of Listeria monocytogenes and E. coli O157. Using the ion exchange chroma﹣tography and size exclusion chromatography, crude peptides from Xuanwei ham were extracted and puri﹣fied to detect the antibacterial activity and the sequences of peptides with strong antibacterial activity were further identified with LC﹣MC technology. The results indicated that Xuanwei ham crude peptides signifi﹣cantly inhibited the growth of Listeria monocytogenes and E. coli O157. Depending on the purification by ion exchange chromatography, the crude peptides were separated to 7 fractions and the 7th fraction had better antibacterial activities with the inhibition rate of 98% and 56% on E. coli O157 and Listeria mono﹣cytogenes, respectively. The peptide sequences of the 7th fraction were identified as QYYNGEEHVRFDS﹣DVGEYR, LRNLPNLEVLDLGTNFI and FASFEAQGALANIAVDK by Nano﹣LC﹣ESI﹣MS/MS. The three identified peptides were synthesized and also exerted stable antibacterial activity, and the inhibition effect on E. coli O157 was significantly higher than that of Listeria monocytogenes. The current results indicated that antibacterial peptides were existed in Xuanwei ham, which provided a theoretical basis for explaining the functional characteristics of the peptides in Xuanwei ham.关键词
宣威火腿/粗提多肽/抗菌活性/分离/纯化/结构鉴定Key words
Xuanwei ham/ crude peptides/ antibacterial activity/ isolation/ purification/ structure iden﹣tification分类
轻工纺织引用本文复制引用
邢路娟,曹松敏,郑锦晓,周光宏,张万刚..宣威火腿中抗菌肽的分离、纯化及鉴定[J].食品科学技术学报,2019,37(6):46-52,7.基金项目
国家重点研发计划项目(2018YFD0400101). （2018YFD0400101）
