食品科学技术学报2019，Vol.37Issue(6)：53-63,11.DOI:10. 3969/j. issn. 2095﹣6002. 2019. 06. 008
5﹣十七烷基间苯二酚对三阴性乳腺癌MDA﹣MB﹣231细胞增殖的抑制作用
Anti﹣Proliferative Effect of 5﹣Heptadecylresorcinol on MDA﹣MB﹣231 Triple﹣Negative Breast Cancer Cells
摘要
Abstract
The effect of 5﹣heptadecylresorcinol ( AR﹣C17) on human breast cancer MDA﹣MB﹣231 cells and its underlying mechanisms were investigated. CCK﹣8 assay was used to detect the change of cell viability under different treatments. DCF﹣DA fluorescence probe assay was used to detect reactive oxygen species ( ROS) changes. Flow cytometry was applied to measure the expression of intracellular tumor proliferation marker Ki﹣67, cell apoptosis changes, mitochondrial potential changes and autophagosome formation. Western blotting was used to detect the expression of related proteins including Bax, Bcl﹣2, LC3﹣Ⅱ. Compared with control group, AR﹣C17 treatment significantly decreased MDA﹣MB﹣231 cells viability and the expression of Ki﹣67, a marker of tumor proliferation. Furthermore, AR﹣C17 treatment could induce cells apoptosis by inducing intracellular ROS, reducing mitochondrial membrane potential, upregulating Bax/Bcl﹣2 ratio as well as increasing actived Caspase. Meanwhile, AR﹣C17 activated autophagy by increasing LC3﹣Ⅱ expression level and autophagosomes formation in cytoplasm. Inhibition of autophagy could further reduce cell viability and enhanced AR﹣C17﹣induced cell apoptosis. AR﹣C17 could inhibit proliferation of human triple﹣negative breast cancer MDA﹣MB﹣231 cells through increasing cell apoptosis and autophagy.关键词
5﹣十七烷基间苯二酚/乳腺癌/凋亡/自噬/增殖抑制/细胞毒性Key words
5﹣heptadecylresorcinol/ breast cancer/ apoptosis/ autophagy/ proliferation inhibition/ cyto﹣toxicity分类
轻工纺织引用本文复制引用
解明思,刘洁,朱科学,孙宝国,王静..5﹣十七烷基间苯二酚对三阴性乳腺癌MDA﹣MB﹣231细胞增殖的抑制作用[J].食品科学技术学报,2019,37(6):53-63,11.基金项目
国家自然科学基金资助项目(31701640) （31701640）
北京市优秀人才培养资助青年拔尖团队项目(2016000026833TD01) （2016000026833TD01）
北京市属高校高水平教师队伍建设支持计划项目(IDHT20180506). （IDHT20180506）
