食品科学技术学报2019，Vol.37Issue(6)：81-87,7.DOI:10. 3969/j. issn. 2095﹣6002. 2019. 06. 011
石斛组6种石斛主要化学成分差异分析
Analysis of Main Chemical Ingredients in Six Species of Dendrobium in Sect. Dendrobium
摘要
Abstract
The contents of polysaccharides, alcohol﹣soluble constituents, Dendrobium alkaloids and amino acids in six species of Dendrobium in sect. Dendrobium were compared, which provided scientific basis for the identification, classification and medicinal application of Dendrobium germplasm resources. Six species of Dendrobium in sect. Dendrobium were collected and the contents of polysaccharide, alco﹣hol﹣soluble constituents, Dendrobium alkaloids and 17 amino acids were measured by sulfuric acid﹣phenol method, hot dipping method, gas chromatography and acid hydrolysis method, respectively. The contents of polysaccharides, alcohol﹣soluble constituents, Dendrobium alkaloids and amino acids in stem of six species of Dendrobium were significantly different. The content of polysaccharide ranged from 6. 92% to 26. 90%, among which the content of polysaccharides in Dendrobium fimbriatum was the highest. The content of alcohol soluble composition was 3. 82% ~13. 01%, and the highest content was found in Den﹣drobium aurantiacum. Meanwhile, all Dendrobium were higher than the content in Chinese Pharmacopoe﹣ia except Dendrobium fimbriatum. Only Dendrobium aurantiacum contained dendrobine, and the content was 0. 019% which was lower than that in the Chinese Pharmacopeia. All Dendrobium contained plentiful amino acid, the contents of total amino acids were in the range of 3. 18% ~8. 09%. Amino acid princi﹣pal component analysis showed that the first five main components contained 17 amino acids, which could be used as descriptive indicators of functional amino acids, serine, tyrosine and phenylalanine were the characteristic amino acids of Dendrobium in sect. Dendrobium. The correlation analysis of chemical com﹣positions showed that the content of polysaccharide was negatively correlated with the alcohol soluble com﹣ponents and the total amino acids. There was a positive correlation between alcohol soluble components and amino acids. There were evident differences among the main chemical content of six species of Den﹣drobium in sect. Dendrobium, suggesting that the pharmacological action of six species of Dendrobium was different and the production application should be carried out according to their compositions and contents.关键词
石斛/多糖/醇溶性成分/石斛碱/氨基酸/资源开发Key words
Dendrobium/ polysaccharide/ alcohol﹣soluble constituents/ Dendrobium alkaloids/ amino acids/ resource exploitation分类
轻工纺织引用本文复制引用
王晓媛,王彦兵,李泽生,李标,丁刚,白燕冰..石斛组6种石斛主要化学成分差异分析[J].食品科学技术学报,2019,37(6):81-87,7.基金项目
农业农村部物种品种资源保护(热带作物)项目(15181301354052710). （热带作物）
