食品科学技术学报2019，Vol.37Issue(6)：100-107,8.DOI:10. 3969/j. issn. 2095﹣6002. 2019. 06. 014
玉米黄粉二步酶解制备高F值寡肽的工艺优化
Optimization on Preparation of High Fischer Ratio Oligopeptides by Two﹣Step Enzymolysis from Corn Yellow Powder
摘要
Abstract
The effects of two﹣step enzymolysis of corn yellow powder on the preparation of high fischer ratio ( F value) oligopeptides were studied. The results showed that the protease type, enzyme addition amount, solid﹣liquid ratio, pH, enzymatic temperature and reaction time had certain influences on the protein conversion rate and F value of active peptides. The primary enzymolysis conditions obtained by the single factor and orthogonal test optimization were as follows: alkaline protease amount 12. 5 kU/g (based on corn yellow powder mass), pH 9. 0, solid﹣liquid ratio 1∶35 g/mL, enzymatic temperature 50℃, and reaction time 3. 0 h. The protein conversion rate reached 85. 61%. Using first﹣step enzymatic so﹣lution as raw material, the conditions of secondary enzymolysis optimized by the single factor and orthogo﹣nal test were obtained as follows: complex flavor protease amount 2. 7 kU/mL ( based on volume of the first﹣step enzymatic solution), pH 7. 0, enzymatic temperature 45 ℃, and reaction time 2. 5 h. After two﹣step enzymolysis of corn yellow powder, the F value of the samples adsorbed by activated carbon reached 28. 30, and the oligopeptide yield was 35%. The molecular weight of oligopeptides were between 493. 46-912. 84 Da and high fischer ratio oligopeptides made up more than 85%. It provides theoretical and technical guidance for the comprehensive utilization of corn yellow powder resources.关键词
玉米黄粉/二步酶解/高F值寡肽/碱性蛋白酶/复合风味蛋白酶Key words
corn yellow powder/ two﹣step enzymolysis/ high fischer ratio oligopeptide/ alkaline protease/complex flavor protease分类
轻工纺织引用本文复制引用
杨华青,侯威,赵磊,王成涛,卢松..玉米黄粉二步酶解制备高F值寡肽的工艺优化[J].食品科学技术学报,2019,37(6):100-107,8.基金项目
国家重点研发计划项目( 2016YFD0400802 ) （ 2016YFD0400802 ）
北京市属高校高水平教师队伍建设支持计划项目( CIT&TCD201704042 （）
IDHT20180506) （）
北京市科技创新服务能力建设-科技成果转化提升计划项目(PXM2018-014213-000033) （PXM2018-014213-000033）
2019年基本科研经费资助项目(PXM2019-014213-000007) （PXM2019-014213-000007）
人才培养质量建设-一流专业建设资助项目(PXM2019-014213-000010). （PXM2019-014213-000010）
