食品科学2019，Vol.40Issue(24)：157-166,10.DOI:10.7506/spkx1002-6630-20181209-103
冷榨花生粕蛋白胨酶法制备及其在微生物增殖培养中的应用
Enzymatic Preparation of Peptone from Cold-Pressed Peanut Meal and Its Application in Microbial Culture
摘要
Abstract
In this study,cold-pressed peanut meal hydrolysate (also named as cold-pressed peanut meal peptone,CPMP) was prepared by sequential enzymatic hydrolysis using pepsin and alkali proteinase.The molecular mass distribution,proximate analysis and amino acid composition of CPMP were determined.Furthermore,its potential as an alternative to the commercial peptone in microbial culture was evaluated.CPMP was mainly composed of ＜ 5.0 kDa peptide fractions.CPMP was rich in Ala,Arg,Asp,and Cys,especially Glu.Escherichia coli grew better for 8 h in CPMP medium than in beef peptone yeast medium.Bacillus subtilis exhibited a longer lag phase in CPMP medium than in Luria-Bertani medium and possessed a better growth behavior after 8 h incubation.The pH of CPMP medium inoculated with E.coii or B.subtilis displayed no statistically significant difference compared with the control medium (P ＞ 0.05).Lactobacillus casei exhibited a different growth behavior in CPMP medium from that in MRS medium (P ＜ 0.05).Staphylococcus aureus grew well in CPMP medium with no significant difference from the control.Saccharomyces cerevisiae in CPMP showed similar growth behavior and pH trend to those in the control.Aspergillus oryzae grew well in CPMP,similar to that in Czapek-Dox medium.Our evidence demonstrated that CPMP has a good potential as a commercial peptone alternative for microbial growth.关键词
冷榨花生粕/蛋白胨替代品/协同酶解/微生物增殖培养Key words
cold-pressed peanut meal/peptone alternative/synergistic hydrolysis/microbial culture分类
轻工纺织引用本文复制引用
肖怀秋,李玉珍,林亲录,刘军,兰立新..冷榨花生粕蛋白胨酶法制备及其在微生物增殖培养中的应用[J].食品科学,2019,40(24):157-166,10.基金项目
湖南省自然科学基金科教联合基金项目(2017JJ5020) （2017JJ5020）
