食品科学2021，Vol.42Issue(16)：224-232,9.DOI:10.7506/spkx1002-6630-20200529-355
免疫磁分离和可视化金纳米杂交探针法快速检测婴幼儿配方乳粉中的阪崎克罗诺杆菌
Rapid Detection of Cronobacter sakazakii in Powdered Infant Formula by Immunomagnetic Separation Coupled with Visual Nanogold Hybridization Probe
摘要
Abstract
A rapid and sensitive assay for detecting Cronobacter sakazakii in powdered infant formula (PIF) was developed by utilizing immunomagnetic separation (IMS) combined with visual gold nanoparticle (GNP) hybridization probe.C.sakazakii was captured via antibody-functionalized magnetic particles (MPs) and the polymerase chain reaction amplicon of the target bacteria was analyzed by GNP instead of traditionally used electrophoresis.Immunomagnetic particles (IMPs)were obtained by mixing together 100 μL of MPs,120 μL of carbodiimide hydrochloride (EDC) and 80 μL of monoclonal antibody against C.sakazakii with 0.02％ (V/V) added Tween-20.Specificity test showed positive results only for C.sakazakii.C.sakazakii could be detected at 102 CFU/mL and 103 CFU/g in pure culture and artificially contaminated PIF without pre-enrichment,respectively.After 3 h of pre-enrichment,this assay was able to detect as low as 4.5 × 101 CFU/g of C.sakazakii in PIF.The results of the visual assay were consistent with those of electrophoresis and ultraviolet scanning spectroscopy.Therefore,the IMS-GNP probe assay proved to be an alternative to gel electrophoresis for the rapid detection of C.sakazakii in PIF samples.关键词
阪崎克罗诺杆菌/免疫磁分离/金纳米颗粒/婴幼儿配方乳粉/可视化检测Key words
Cronobacter sakazakii/immunomagnetic separation/gold nanoparticles/powdered infant formula/visual detection分类
轻工纺织引用本文复制引用
姜毓君,任玉伟,张微,杨涛,满朝新,周文琦,王蕊,陈思涵,杨鑫焱,付世骞,高平娉..免疫磁分离和可视化金纳米杂交探针法快速检测婴幼儿配方乳粉中的阪崎克罗诺杆菌[J].食品科学,2021,42(16):224-232,9.基金项目
国家自然科学基金面上项目(31871828) （31871828）
