食品科学2023，Vol.44Issue(16)：301-311,11.DOI:10.7506/spkx1002-6630-20220914-126
基于UPLC-MS/MS方法分析绿茶非挥发性代谢物在常温贮藏中的动态变化
Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry Analysis of Dynamic Changes in Non-volatile Compounds in Green Tea during Storage at Ambient Temperature
摘要
Abstract
Herein,we used an ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)system to investigate changes in the profile of non-volatile metabolites in green tea after different periods(15,40,80 and 120 days)of storage at 37℃.The color of green tea and tea infusion changed after 40 days of storage at 37℃when compared with the control(stored at-80℃).During 40 days of storage,the content of catechins did not significantly change,but the content of free amino acids significantly decreased.A total of 684 non-volatile components were identified,77 of which were found to be characteristic differential metabolites,the major ones being polyphenols,lipids and organic acids.The content of octadecadien-6-ynoic acid,an umami-related compound,significantly decreased after 40 days and was undetected after 80 days.The content of theaflavine-3-catechin,related to the color and taste of tea infusion,significantly increased with storage time.Similarly,the content of flavone glycoside increased with storage time.Moreover,the content of lipids such as triacylglycerols(TAGs)and diacylglycerol(DAGs)changed significantly after 15 days,and the content of free fatty acids increased with storage time.The findings from this study showed that the contents of lipids and flavonoids in green tea significantly changed during storage,which played an important role in the quality deterioration of green tea.关键词
绿茶/贮藏/非挥发性代谢物/超高效液相色谱-串联质谱联用技术/类黄酮代谢/脂质代谢Key words
green tea/storage/non-volatile compounds/ultra-high performance liquid chromatography-tandem mass spectrometry/flavonoids/lipids分类
农业科学引用本文复制引用
周汉琛,王辉,刘亚芹,雷攀登..基于UPLC-MS/MS方法分析绿茶非挥发性代谢物在常温贮藏中的动态变化[J].食品科学,2023,44(16):301-311,11.基金项目
国家自然科学基金青年科学基金项目(32002096) （32002096）
安徽省农业科学院青年英才计划项目(QNYC-202119) （QNYC-202119）
