A systematic identification of cold tolerance genes in peanut using yeast functional screening systemOACSCD
Peanut(Arachis hypogaea L.)is a thermophilic crop,and low temperature leads to a significant reduction in annual yields.Despite a few cold tolerant germplasms or cultivars have been discovered and developed,molecular mechanisms governing peanut cold tolerance is poorly understood.Identification of keys genes involved in cold tolerance is the first step to address the underlying mechanism.In this study,we isolated and characterized 157 genes with potentials to confer cold tolerance in peanut by using a yeast functional screening system.GO(Gene ontology)and KEGG(Kyoto encyclopedia of genes and genomes)enrichment analysis of these genes revealed that ribosome and photosynthesis proteins might play essential roles in peanut cold response.Transcriptome results indicated that 60 cold tolerance candidate genes were significantly induced or depressed by low temperature.qRT-PCR analysis demonstrated that several candidate genes could be also regulated by salt or drought stress.Individual overexpression of two UDP-glycosyltransferases(AhUGT2 and AhUGT268)in transgenic yeast cells could enhance their tolerance to multiple abiotic stress.In conclusion,this study advances our understanding of the mechanisms associated with the cold stress responses in peanut,and offers valuable gene resources for genetic improvement of abiotic stress tolerance in crops.
Xin Wang;Yue Liu;Ruonan Yao;Lei Ouyang;Tingting Yu;Liying Yan;Yuning Chen;Dongxin Huai;Zhihui Wang;Yanping Kang;Qianqian Wang;Huifang Jiang;Yong Lei;Boshou Liao
Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs,Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences,Wuhan,430062,PR ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs,Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences,Wuhan,430062,PR ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs,Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences,Wuhan,430062,PR China State Key Laboratory of Biocatalysis and Enzyme Engineering,School of Life Sciences,Hubei University,Wuhan,430062,PR ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs,Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences,Wuhan,430062,PR China State Key Laboratory of Biocatalysis and Enzyme Engineering,School of Life Sciences,Hubei University,Wuhan,430062,PR ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs,Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences,Wuhan,430062,PR ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs,Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences,Wuhan,430062,PR ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs,Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences,Wuhan,430062,PR ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs,Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences,Wuhan,430062,PR ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs,Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences,Wuhan,430062,PR ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs,Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences,Wuhan,430062,PR ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs,Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences,Wuhan,430062,PR ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs,Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences,Wuhan,430062,PR ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs,Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences,Wuhan,430062,PR ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs,Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences,Wuhan,430062,PR China
农业科学
PeanutYeast screening systemCold toleranceUDP-glycosyltransferaseAbiotic stress
《Oil Crop Science-中国油料作物学报英文版》 2023 (3)
P.184-190,7
This work was supported by a grant from the National Natural Science Foundation of China(No.32170278)the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences(No.CAAS-ASTIP-2021-OCRI)the Earmarked fund for China Agricultural Research System(CARS-13).
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