食品科学2023，Vol.44Issue(21)：23-34,12.DOI:10.7506/spkx1002-6630-20230430-291
体外消化对β-胡萝卜素大豆分离蛋白纳米颗粒黏液层渗透及跨膜转运的影响机制
Influence Mechanism of in Vitro Digestion Process on Mucus Layer Permeation and Transmembrane Transport of Soy Protein Isolate Nanoparticles Loaded with β-Carotene
摘要
Abstract
The in vivo absorption of nanocarriers is a complex process,which is affected by the bioabsorption barrier.It is doubtful whether nanopreparations can promote the absorption and utilization of bioactive molecules.In this study,soy protein isolate(SPI)was used to prepare plant-based nanocarriers encapsulating β-carotene,and an in vitro digestion model was employed to investigate the structural changes of β-carotene loaded soy protein isolate nanoparticles(BC-SPIs)during digestion.Additionally,the Caco2 cell transport model was used to explore the impact mechanism of digestion conditions on the transmembrane transport of BC-SPIs after digestion.Furthermore,a Caco2-HT29 co-culture model containing a mucus layer was used to investigate the permeability of BC-SPIs through the mucus layer before and after digestion.This study found that before digestion,BC-SPIs could be directly absorbed by the Caco-2 cell monolayer through clathrin-and caveolin-dependent endocytosis.After in vitro simulated digestion,the size of BC-SPIs increased,and they could be directly absorbed by cells through clathrin-dependent endocytosis,caveolin-dependent endocytosis and macropinocytosis.The digested nanoparticles carried more negative charge,resulting in a 1.48-fold enhancement in the ability to cross the mucus layer and a 1.56-fold increase in the transmembrane transport efficiency of β-carotene.This study clarified the different absorption pathways of BC-SPIs before and after digestion and revealed the interaction between BC-SPIs and bile salts under simulated digestion conditions and the contribution of increasing the size of BC-SPIs to their transport and absorption efficiency by cells.These findings lay a theoretical foundation for further improving the application potential of nanocarriers in bioavailability enhancement,which will help advance the development of nanotechnology in several fields such as pharmaceuticals and health products.关键词
纳米载体/大豆分离蛋白/跨膜转运/黏液层渗透/细胞吸收Key words
nanocarriers/soy protein isolate/transmembrane transport/mucus layer permeation/cellular uptake分类
轻工纺织引用本文复制引用
陈羚,吕园,徐菲菲,钟芳..体外消化对β-胡萝卜素大豆分离蛋白纳米颗粒黏液层渗透及跨膜转运的影响机制[J].食品科学,2023,44(21):23-34,12.基金项目
国家自然科学基金青年科学基金项目(32302282) （32302282）
国家自然科学基金面上项目(32072153) （32072153）
江苏省自然科学基金青年科学基金项目(BK20210460) （BK20210460）
中国博士后科学基金面上项目(2021M700057) （2021M700057）
