食品科学2023，Vol.44Issue(21)：131-136,6.DOI:10.7506/spkx1002-6630-20221009-068
紫薯花青素通过调节p53-p21Waf1/Cip1信号通路对辐射致造血干/祖细胞衰老的保护作用
Protective Effect of Solanum tuberosum Anthocyanin against Radiation-Induced Hematopoietic Stem/Progenitor Cell Senescence via the p53-p21Waf1/Cip1 Pathway
摘要
Abstract
Objective:To investigate the molecular mechanism underlying the protective effect of Solanum tuberosum anthocyanin(STA)on radiation-induced hematopoietic stem/progenitor cell senescence.Methods:C57BL/6 mice were randomly divided into three groups:control,model,STA treatment and STA prevention,and the cell senescence model was constructed by X-ray irradiation.The hemogram of mice in each group were examined using a hematology analyzer after drug administration.Stem cell antigen 1 positive hematopoietic stem/progenitor cells(Sca-1+HSC/HPCs)were isolated and purified from each group by immunomagnetic cell sorting and were stained using a senescence-associated β-galactosidase(SA-β-Gal)kit to calculate the proportion of SA-β-Gal positive cells.The number of mixed lineage colony forming unit(CFU-Mix)was used to evaluate the colony-forming capacity and differentiation potential of hematopoietic stem/progenitor cells.Cell cycle was analyzed by flow cytometry.The mRNA and protein expression of p53 and p21Waf1/Cip1 were detected by quantitative real-time polymerase chain reaction(qPCR)and Western blot.Results:The numbers of red blood cells(RBCs),white blood cells(WBCs)and platelets in the peripheral blood were significantly lower in the model group than in the control group(P<0.01).In addition,compared with the control group,the proportion of SA-β-Gal positive cells significantly increased(P<0.01),the number of CFU-Mix significantly fell(P<0.01),the proportion of G0/G1 phase cells significantly increased,the proportions of G2/M and S phase cells significantly dropped(P<0.01),and the relative mRNA and protein expression levels of p53 and p21Waf1/Cip1 significantly increased in the model group(P<0.01).STA could significantly restore the above parameters of mice in the model group irrespective of whether it was administered before or after X-ray radiation,with more pronounced effect being observed in the former case.Conclusion:STA can protect hematopoietic stem/progenitor cells against radiation by regulating the p53-p21Waf1/Cip1 pathway.关键词
紫薯花青素/p53-p21Waf1/Cip1信号通路/辐射/造血干/祖细胞/衰老Key words
Solanum tuberosum anthocyanin/p53-p21Walf1/Cip1 pathway/radiation/hematopoietic stem/progenitor cell/senescence分类
医药卫生引用本文复制引用
陈彩云,王若宇,张家乐,张怡明,石塔拉,宓伟..紫薯花青素通过调节p53-p21Waf1/Cip1信号通路对辐射致造血干/祖细胞衰老的保护作用[J].食品科学,2023,44(21):131-136,6.基金项目
国家自然科学基金青年科学基金项目(81302426 （）
81902161) （）
山东省高等学校科技计划项目(J17KA224) （J17KA224）
山东省医药卫生科技发展计划项目(202212020325 （）
202012020832) （）
