中医康复2024，Vol.1Issue(1)：21-25,31,6.DOI:10.19787/j.issn.2097-3128.2024.01.005
推拿对骨骼肌过度使用损伤修复中Hippo信号通路的影响
Tuina Promotes Muscle Injury Repair in Exercised-induced Muscle Injury Rats by Regulating Hippo Signaling Pathway
摘要
Abstract
Objective:To explore the repair effect of tuina on overuse-induced skeletal muscle injury in rats and the effects of Hippo signaling path-way and myogenic regulatory factors.Methods:Twenty-four male SD rats were randomly divided into control group(C group),model group(M group),model + tuina group(T group)and model + sham tuina group(G group),with 6 rats in each group.EIMD model was established by 4-week heavy load exercise in group M,T,G;Group T was treated with tuian by pressing 6 hours after exercise,and Group G was treated with brush at the same time.Ultrastructural changes of muscle fibers were observed by transmission electron microscope,cross-sectional area of muscle fibers was evaluated by HE staining,mRNA expression of MST1,YAP,TAZ,TEAD2 was detected by real-time quantitative RT-PCR,and expression of PAX7,MYOD,MYOG protein was detected by Western Blot.Results:(1)Behavioral results showed that the ratio of grasping force/body mass of limbs in-creased and CSA decreased in model group and sham push group(P<0.05).Tuina could further increase the ratio of grasping force/body mass of limbs and increase CSA(P<0.05);Transmission electron microscope showed that muscle fiber sarcomere swelling,Z-line rupture and cell arrange-ment disorder in model group and sham tuina group.Tuina promoted muscle fiber repair to a certain extent;The expression of YAP,TAZ,TEAD2 mRNA decreased and the expression of MST1 mRNA increased in model group and sham tuina group,but the expression of YAP,TAZ,TEAD2 mRNA increased and the expression of MST1 mRNA decreased(P<0.05);The expression of PAX7,MYOD protein decreased and the expression of MYOG protein increased in model group and sham tuina group.Tuina increased the expression of PAX7,MYOD protein and decreased the expres-sion of MYOG protein.Conclusion:tuina can effectively promote the benign repair of overused muscle injury,and the mechanism may be related to the regulation of hippo signaling pathway and myogenic regulatory factors.关键词
推拿/运动性骨骼肌损伤/Hippo信号通路/生肌调节因子Key words
Tuina/overuse skeletal muscle injury/Hippo signaling pathway/myogenic regulatory factor分类
医药卫生引用本文复制引用
阿衣留布,靳松林,李伦宇,丁海丽..推拿对骨骼肌过度使用损伤修复中Hippo信号通路的影响[J].中医康复,2024,1(1):21-25,31,6.基金项目
国家自然科学基金(NO.81904318) （NO.81904318）
