推拿对骨骼肌过度使用损伤修复中Hippo信号通路的影响OA

Objective:To explore the repair effect of tuina on overuse-induced skeletal muscle injury in rats and the effects of Hippo signaling path-way and myogenic regulatory factors.Methods:Twenty-four male SD rats were randomly divided into control group(C group),model group(M group),model + tuina group(T group)and model + sham tuina group(G group),with 6 rats in each group.EIMD model was established by 4-week heavy load exercise in group M,T,G;Group T was treated with tuian by pressing 6 hours after exercise,and Group G was treated with brush at the same time.Ultrastructural changes of muscle fibers were observed by transmission electron microscope,cross-sectional area of muscle fibers was evaluated by HE staining,mRNA expression of MST1,YAP,TAZ,TEAD2 was detected by real-time quantitative RT-PCR,and expression of PAX7,MYOD,MYOG protein was detected by Western Blot.Results:(1)Behavioral results showed that the ratio of grasping force/body mass of limbs in-creased and CSA decreased in model group and sham push group(P<0.05).Tuina could further increase the ratio of grasping force/body mass of limbs and increase CSA(P<0.05);Transmission electron microscope showed that muscle fiber sarcomere swelling,Z-line rupture and cell arrange-ment disorder in model group and sham tuina group.Tuina promoted muscle fiber repair to a certain extent;The expression of YAP,TAZ,TEAD2 mRNA decreased and the expression of MST1 mRNA increased in model group and sham tuina group,but the expression of YAP,TAZ,TEAD2 mRNA increased and the expression of MST1 mRNA decreased(P<0.05);The expression of PAX7,MYOD protein decreased and the expression of MYOG protein increased in model group and sham tuina group.Tuina increased the expression of PAX7,MYOD protein and decreased the expres-sion of MYOG protein.Conclusion:tuina can effectively promote the benign repair of overused muscle injury,and the mechanism may be related to the regulation of hippo signaling pathway and myogenic regulatory factors.