中医康复2024，Vol.1Issue(1)：32-35,4.DOI:10.19787/j.issn.2097-3128.2024.01.007
柱前衍生化结合气质联用技术测定实验性高脂血症大鼠血浆中代谢标志物
Determination of Metabolic Biomarkers in Plasma of Experimental Hyperlipidemia Rat Model by Pre-Column Derivatization Combined with GC-MS
摘要
Abstract
Objective:By pre-column derivatization to establish a gas chromatography-mass spectrography method for determination of protential metabolic biomarkers,and was used to study the metabolic changes in the plasma of experimental hyperlipidemia rats.Methods:SD rats were ran-domly divided into normal control group and experimental hyperlipidemia model group.The experimental hyperlipidemia rat model was reproduced by feeding with high-fat diet.Orbital blood was taken and pre column derivatization was performed.The analytical method was established by GC-MS and investigated for methodology,including precision,reproducibility and stability.The plasma samples of normal control group and experimen-tal hyperlipidemia group were detected,and the potential metabolic markers were identified.Results:A metabonomic method based on GC-MS was established for the analysis of experimental hyperlipidemia plasma.The RSD of the relative peak areas in the precision,repeatability and stability tests of the method were less than 10%.Conclusion:The method is rapid,accurate,reproducible,and can be used for the determination of endog-enous metabolites in plasma of rats with hyperlipidemia.The method can provide some reference for the application of metabonomics in hyperlipid-emia.关键词
气质联用技术/高脂血症/代谢组学/代谢标志物/大鼠Key words
GC-MS/hyperlipidemia model/metabonomics/metabolic biomarkers/rat分类
医药卫生引用本文复制引用
徐文杰..柱前衍生化结合气质联用技术测定实验性高脂血症大鼠血浆中代谢标志物[J].中医康复,2024,1(1):32-35,4.基金项目
广东省自然科学基金自由申请项目(2017A030313628) （2017A030313628）
广东省科技计划项目(2017A070701017) （2017A070701017）
