柱前衍生化结合气质联用技术测定实验性高脂血症大鼠血浆中代谢标志物OA

Objective:By pre-column derivatization to establish a gas chromatography-mass spectrography method for determination of protential metabolic biomarkers,and was used to study the metabolic changes in the plasma of experimental hyperlipidemia rats.Methods:SD rats were ran-domly divided into normal control group and experimental hyperlipidemia model group.The experimental hyperlipidemia rat model was reproduced by feeding with high-fat diet.Orbital blood was taken and pre column derivatization was performed.The analytical method was established by GC-MS and investigated for methodology,including precision,reproducibility and stability.The plasma samples of normal control group and experimen-tal hyperlipidemia group were detected,and the potential metabolic markers were identified.Results:A metabonomic method based on GC-MS was established for the analysis of experimental hyperlipidemia plasma.The RSD of the relative peak areas in the precision,repeatability and stability tests of the method were less than 10%.Conclusion:The method is rapid,accurate,reproducible,and can be used for the determination of endog-enous metabolites in plasma of rats with hyperlipidemia.The method can provide some reference for the application of metabonomics in hyperlipid-emia.