中国食品学报2023，Vol.23Issue(11)：13-23,11.DOI:10.16429/j.1009-7848.2023.11.002
miR-642a-3p对藻蓝色素抑制非小细胞肺癌活性的调控作用
The Regulation Function of miR-642a-3p on the Inhibitory Activity of Non-small Cell Lung Cancer Mediated by Phycocyanin
摘要
Abstract
Phycocyanin(PC)is a kind of natural pigment protein complex derived from marine algae.It is also a type of important food functional factor with anti-cancer,anti-inflammatory,and immune-enhancing activities,and has been widely used in functional food,medicine and cosmetics.Phycocyanin has been proven to inhibit the activity of non-small cell lung cancer(NSCLC)cells,but the underlying regulatory mechanism is not fully understood.This study focused on miR-642a-3p,and investigated the potential regulating mechanism of phycocyanin in NSCLC cells.The results showed that phycocyanin upregulated the expression level of miR-642a-3p in four NSCLC cells(A549,H1299,H460 and LTEP-a2).Overexpression of miR-642a-3p levels significantly inhibited the proliferation,migration and unanchored growth of four cells in vitro.In addition,transfection of miRNA inhibitors to interfere with the expression of miR-642a-3p could enhance cell viability to a certain extent.This study preliminarily revealed that the up-regulation expression of miR-642a-3p was involved in the regulation of inhibiting NSCLC cell viabilities by phycocyanin,which could lay a sci-entific theoretical basis for the in-depth study and utilization of phycocyanin.关键词
藻蓝色素/miR-642a-3p/非小细胞肺癌/细胞活性Key words
phycocyanin/miR-642a-3p/non-small cell lung cancer/cell viability引用本文复制引用
李前程,郝帅,李爽,刘媛璞,李凡念,杨起,张文静..miR-642a-3p对藻蓝色素抑制非小细胞肺癌活性的调控作用[J].中国食品学报,2023,23(11):13-23,11.基金项目
国家自然科学基金面上项目(32072231,32272321) （32072231,32272321）
北京市属高等学校优秀青年人才培育计划项目(BPHR202203042) （BPHR202203042）
