中国食品学报2023，Vol.23Issue(11)：24-35,12.DOI:10.16429/j.1009-7848.2023.11.003
鼠李糖乳杆菌Probio-M9连续传代过程中的遗传稳定性
Genetic Stability of Lacticaseibacillus rhamnosus Probio-M9 during Continuous Subculturing
摘要
Abstract
Objective:To investigate the genetic stability of Lacticaseibacillus rhamnosus Probio-M9(L.rhamnosus Pro-bio-M9)during continuous passage.Methods:The morphological changes,carbohydrate utilization capacity,activity and metabolic characteristics of L.rhamnosus Probio-M9 were compared in the 0,25,50,75 and 100 passages of the pro-biotic strain.At the same time,comparative genomics was used to analyze the changes of genome level during the con-tinuous culture of 100 generations.Results:There were no significant changes(P＞0.05)in phenotypic characteristics and metabolic characteristics of L.rhamnosus Probio-M9 during 100 generations of continuous culture,and the phenotyp-ic characteristics were stable,and the strain activity was maintained well.At the same time,using the genome of L.rhamnosus Probio-M9 as the reference sequence,15 strains of different generations were sequenced,and the genome size and GC content showed no significant difference between the 15 strains cultured continuously and L.rhamnosus Probio-M9.Phylogenetic relationships showed that the 15 strains were closely related to the original genome.From the identified SNP loci,no mutation locus with stable inheritance was found in every two generations,and the collinear strain was good.The mutation level of the coding gene at the genome level confirmed that L.rhamnosus Probio-M9 had stable phe-notypic characteristics and metabolic characteristics during the continuous culture of 100 generations.Conclusion:L.rhamnosus Probio-M9 has good genetic stability during continuous passage,which provides a theoretical basis for further development,research and industrialization of L.rhamnosus Probio-M9.关键词
鼠李糖乳杆菌Probio-M9/连续传代/表型/代谢/比较基因组学/遗传稳定性Key words
Lacticaseibacillus rhamnosus Probio-M9/continuous subculturing/phenotype/metabolism/comparative ge-nomics/genetic stability引用本文复制引用
黄天,刘凯龙,刘晓晔,姚国强,孙天松..鼠李糖乳杆菌Probio-M9连续传代过程中的遗传稳定性[J].中国食品学报,2023,23(11):24-35,12.基金项目
财政部和农业农村部:国家现代农业产业技术体系资助项目 （）
