中国食品学报2023，Vol.23Issue(11)：36-45,10.DOI:10.16429/j.1009-7848.2023.11.004
利用低聚半乳糖双歧杆菌的筛选及益生特性研究
Studies on Screening and Probiotic Characterization of Bifidobacteria with the Ability to Utilize Galacto-oligogalactosaccharides
摘要
Abstract
Short chain-galactooligosaccharides(SC-GOS)is an effective proliferation factor of Bifidobacteria.Seven strains of Bifidobacteria with high utilization of SC-GOS were screened from infant fecal samples,the result of 16S rDNA iden-tification was that one was Bifidobacterium longum subsp.infantis BI_Y46,2 B.pseudocatenulatum(BP_Y43,BP_YA),2 B.bifidum(BB_Y22,BB_S7)and 2 strains of B.longum(BL_S34,BL_H26).Compared with B.infantis M63 and B.infantis ATCC 15697,the morphology of the strains was observed and the probiotic characteristics were analyzed.Thus,the probiotics with application potential were screened.The results showed that BI_Y46 had special morphology with bifurcated fimbriae,and the exopolysaccharide yield of BB_Y22 and BP_Y43 was higher than 0.45 mg/mL.The sur-face protein content of BL_H26 was(0.74±0.07)mg/mL,the content of BB_S7 and BB_Y22 was between 0.35-0.45 mg/mL.Whereas,BP_Y43 and BB_Y22 had strong surface hydrophobicity and self-aggregation ability.BB_Y22 and BI_Y46 showed strong tolerance to the stimulation of bile salt and gastric juice.The supernatant of BI_Y46 and BB_S7 had strong inhibiting effect on Escherichia coli ATCC 25922.In summary,B.infantis BIY46,B.bifidum BB_Y22 and B.pseudocatenulatum BPY43 had good probiotic potential and provide some reference for the development of products containing galactoseoligosaccharides and Bifidobacterium.关键词
双歧杆菌/短链低聚半乳糖/益生特性/肠道耐受/抑菌能力Key words
Bifidobacterium/short-chain galactooligosaccharides/probiotic property/intestinal tolerance/bacteriostatic a-bility引用本文复制引用
孙慧,肖利红,何明雪,徐梓赫,谭泽,张峰瑞,张莉丽,韩建春..利用低聚半乳糖双歧杆菌的筛选及益生特性研究[J].中国食品学报,2023,23(11):36-45,10.基金项目
黑龙江省重点研发计划项目(GA21B015) （GA21B015）
黑龙江省博士后科研启动金项目(LBH-Q2009) （LBH-Q2009）
