中国食品学报2023，Vol.23Issue(11)：74-83,10.DOI:10.16429/j.1009-7848.2023.11.008
姜黄素对羟自由基诱导的肌原纤维蛋白氧化及结构的影响
Effects of Curcumin on Oxidation and Structure of Myofibrillar Protein Induced by Hydroxyl Radical
摘要
Abstract
Pork myofibrillar protein(MP)was selected as the main research material.The Fenton oxidation system was employed to replicate the oxidation conditions of meat items,and curcumin with different concentrations(0,5,10,15,20 μmol/L)was added to it.Changes in amino acid side chain,secondary structure,tertiary structure and solubility of protein were analyzed.To explore the inhibitory effect of curcumin on the oxidation of pork myofibrillar protein at differ-ent concentrations and its specific effect on protein structure.The results showed that curcumin had good free radical scavenging performance and could effectively slow the protein oxidation and structural changes caused by hydroxyl radi-cals,and this inhibition was proportional to the concentration of curcumin in a specific concentration range(5-20 μmol/L).Compared with the oxidation group,the curcumin treatment group significantly inhibited the formation of carbonyl groups and the increase of surface hydrophobicity,the curcumin treatment group of 20 μmol/L had the inhibition rates of 2.62％and 51.78％,respectively(P＜0.05),can also significantly alleviate the decline of sulfhydryl,free amino and sol-ubility(P＜0.05),the inhibition rates were 80.02％,59.34％,70.84％,respectively,and the addition of curcumin can promote the MP tertiary structure unfolding,can also alleviate the secondary structure changes caused by hydroxyl radi-cals.Therefore,curcumin can interact with MP to alleviate its oxidative deterioration and structural changes,and has a good effect on the quality and preservation of meat products.关键词
姜黄素/肌原纤维蛋白/氧化/羟自由基Key words
curcumin/myofibrillar protein/oxidation/hydroxyl radical引用本文复制引用
常海军,伯朝英,石源伟,熊杰,胡渝..姜黄素对羟自由基诱导的肌原纤维蛋白氧化及结构的影响[J].中国食品学报,2023,23(11):74-83,10.基金项目
重庆市教委科学技术研究计划项目(KJQN202000817,KJZD-K202200806) （KJQN202000817,KJZD-K202200806）
重庆市自然科学基金项目(cstc2019jcyj-msxmX0472) （cstc2019jcyj-msxmX0472）
重庆市研究生科研创新项目(CYS23553) （CYS23553）
