中国食品学报2023，Vol.23Issue(11)：352-360,9.DOI:10.16429/j.1009-7848.2023.11.033
水产品中氯霉素酶联免疫吸附分析法的构建与应用
Construction and Application of Enzyme-linked Immunosorbent Assay for Chloramphenicol in Aquatic Products
摘要
Abstract
In this work,chloramphenicol(CAP)as the research object,by optimizing the reaction conditions,an indi-rect competitive enzyme-linked immunosorbent assay(ic-ELISA)for CAP was constructed and applied to the detection of CAP residues in aquatic products.The optimal working conditions of the constructed ic-ELISA were 0.05 μg/well coating amount of CAP-OVA,coating conditions at 4℃ for 14 h,0.5％nonfat milk powder as blocking solution,and the dilu-tion ratio of CAP antibody was 1∶16 000.The results showed that the sensitivity(ICso)of the constructed ic-ELISA method was 4.88 μg/mL,and the detection limit(IC15)was 0.29 μg/mL.The cross-reaction rate of the chloramphenicol structural analogs and similar antibiotics were less than 0.1％.The spiked recovery experiments of 5.0,10.0,20.0 μg/kg in Larimichthys crocea,razor clam and bullfrog were carried out,and the recovery rates were within 83.90％-100.73％,and the intraday and interday coefficients of variation(CV)for this method ranged from 2.28％to 6.96％.The detection results were in good agreement with that of LC-MS/MS.The ic-ELISA of CAP in this study was simple to operate and has high sensitivity.It could be applied to the rapid quantitative detection of CAP residues in various aquatic products,and provide technical reference for ic-ELISA of other small molecule hazards.And it is also an indirect competitive en-zyme-linked immunosorbent assay for the construction of the method provides technical reference.关键词
水产品/氯霉素/残留检测/酶联免疫吸附分析法Key words
aquatic products/chloramphenicol/residue detection/ic-ELISA引用本文复制引用
张彪,蔡丹凤,姚天择,郎一涵,傅炎勇,申屠旭萍,俞晓平..水产品中氯霉素酶联免疫吸附分析法的构建与应用[J].中国食品学报,2023,23(11):352-360,9.基金项目
浙江省重点研发计划项目(2022C02049,2021C02061) （2022C02049,2021C02061）
