作物学报2024，Vol.50Issue(1)：42-54,13.DOI:10.3724/SP.J.1006.2024.34047
烟草生物碱性状的QTL定位
QTL mapping of alkaloids in tobacco
摘要
Abstract
Alkaloids are important chemical components in tobacco.In order to understand the genetic architecture of alkaloids in tobacco and identify major effect loci controlling alkaloids related traits,QTL mapping on tobacco alkaloids was performed.A set of 271 recombinant inbred lines(RIL)were constructed with Y3 and K326 as the parents.The RIL population was planted in Yanhe,Yuxi,Yunnan province and Shilin,Kunming,Yunnan province in 2018,2019,and 2020,respectively.Five alkaloid pheno-types including total plant alkali(TPA),nicotine(NIC),nornicotine(NOR),anabasine(ANAB),and anatabine(ANAT)were measured.A linkage map of 46,129 markers was constructed by genome sequencing of the population.QTL mapping was per-formed by the software QTLNetwork 2.0 which was developed based on the mixed linear model.A total of 15 QTLs with signifi-cant additive effects were identified.The contribution rate of additive effect to the corresponding phenotypes varied from 0.58%to 11.57%.Four major QTLs,qTPA14 for total plant alkali,qNIC14 for nicotine,qANAB14 for anabasine,and qANAT14 for ana-tabine,accounted for more than 10%of phenotypic variation of the corresponding traits,which were located in linkage group 14.Six QTLs with significant additive-by-environment interaction effects were detected,their additive-by-environment interaction effects explained the phenotypic variation of 0.80%-1.81%.Five pairs of QTLs with significant additive-by-additive epistasis effects were detected,accounting for phenotypic variation from 0.15%to 2.31%,while two pairs of QTLs were detected with significant epistasis-by-environment interaction effects,which explaining the proportion of phenotypic variation from 0.81%to 1.16%.The results pave a foundation for further isolation of candidate genes,the dissection of genetic mechanism,and the mo-lecular improvement of tobacco alkaloid traits.关键词
烟草/重组自交系/生物碱/复杂性状/基因与环境互作Key words
tobacco/the recombinant inbred line/alkaloids/complex traits/gene by environment interaction引用本文复制引用
刘颖超,方敦煌,徐海明,童治军,肖炳光..烟草生物碱性状的QTL定位[J].作物学报,2024,50(1):42-54,13.基金项目
本研究由中国烟草总公司[110202101002(JY-02),110202101038(JY-15)]和云南省烟草公司项目(2021530000241013,2022530000241009)资助. This study was supported by the China National Tobacco Company[110202101002(JY-02),110202101038(JY-15)]and the Yunnan Tobacco Company(2021530000241013,2022530000241009). （JY-02）
