作物学报2024，Vol.50Issue(1)：76-88,13.DOI:10.3724/SP.J.1006.2024.33007
玉米转录因子ZmMYB12提高植物抗旱性和低磷耐受性的功能鉴定
Functional identification of maize transcription factor ZmMYB12 to enhance drought resistance and low phosphorus tolerance in plants
摘要
Abstract
Maize usually suffers from various abiotic stresses such as drought,high temperature,high salt,and deficiency of nu-trient elements during growth and development period,which will eventually lead to the decline of yield and quality,resulting in serious agricultural yield reduction.MYB transcription factors are widely distributed in plants and involved in the whole process of plant growth and development and environmental response.Therefore,the screening and identification of MYB transcription factors conferring stress resistance can provide the theoretical basis and technical support for genetic improvement in maize.In this study,a R2R3-MYB family transcription factor gene,ZmMYB12,was cloned from maize materials under drought treatment.This gene showed inducible expression in response to drought,ABA,and PEG treatments.Maize plants with virus induced gene silencing(VIGS)of ZmMYB12 had higher sensitivity to drought and accumulated more reactive oxygen species(ROS),as well as smaller roots.The survival rate of ZmMYB12 silencing plants was lower after re-watering than the wild type plants(WT),indicat-ing that ZmMYB12 was a positive regulator of drought response.Overexpression of ZmMYB12 in Arabidopsis thaliana resulted in less ROS accumulation and more lateral roots than WT,thus elevating drought resistance.After low phosphorus stress,ZmMYB12 overexpression Arabidopsis had more lateral roots and stronger root system,as well as higher contents of chlorophyll and anthocyanin.The content of inorganic phosphorus in ZmMYB12 overexpression Arabidopsis was also higher than WT.In conclusion,this study indicates that ZmMYB12 positively regulates drought response and increases the uptake and utilization of phosphorus in plants,providing an elite gene for maize breeding to increase abiotic resistance.关键词
玉米/MYB转录因子/干旱胁迫/低磷胁迫/根系Key words
maize/MYB/drought stress/low phosphorus stress/root引用本文复制引用
王丽平,王晓钰,傅竞也,王强..玉米转录因子ZmMYB12提高植物抗旱性和低磷耐受性的功能鉴定[J].作物学报,2024,50(1):76-88,13.基金项目
本研究由国家自然科学基金项目(31971825)资助. This study was supported by the National Natural Science Foundation of China(31971825). （31971825）
