马铃薯休眠解除相关基因StNF-YA8的表达分析OACSTPCD

Nuclear factor-Y(NF-Y)is a class of transcription factors widely distributed in eukaryotes.It composed of NF-YA,NF-YB and NF-YC subunits.NF-YA is involved in regulating plant growth and development,and stress response.In this study,qRT-PCR method was used to analyze the expression pattern of 10 StNF-YA genes of potato(Solanum tuberosum)in the process of tuber dormancy release.The results showed that the StNF-YA8 gene was consistently up-regulated in expression during dormancy release in tubers.The StNF-YA8 gene was further cloned(GenBank No.XM_015309982.1),and its ORF was 591 bp,encoding 196 amino acids.The subcellular localization vector of StNF-YA8 protein was constructed,and the results showed that StNF-YA8 protein was localized in the nucleus and cell membrane.Tissue-specific analysis revealed that the StNF-YA8 gene was differentially expressed between different tissues.The results of bioinformatics analysis showed that the potato StNF-YA8 protein contained a conserved CBFB domain,and had the highest evolutionary relationship with wild potato(S.commersonii);StNF-YA8 protein closely interacted with StNF-YB3/5/10/14/17 and StNF-YC1/4/5/8/9 proteins;there were cis-acting elements involved in light response,circadian rhythm control and hormone response in the promoter sequence of this gene.The results can provide a basis for the molecular mechanism analysis of potato StNF-YA8 gene involved in regulating tuber dormancy release.