农业生物技术学报2024，Vol.32Issue(1)：50-59,10.DOI:10.3969/j.issn.1674-7968.2024.01.005
马铃薯休眠解除相关基因StNF-YA8的表达分析
Expression Analysis of the StNF-YA8 Gene Associated with Dormancy Release in Potato(Solanum tuberosum)
摘要
Abstract
Nuclear factor-Y(NF-Y)is a class of transcription factors widely distributed in eukaryotes.It composed of NF-YA,NF-YB and NF-YC subunits.NF-YA is involved in regulating plant growth and development,and stress response.In this study,qRT-PCR method was used to analyze the expression pattern of 10 StNF-YA genes of potato(Solanum tuberosum)in the process of tuber dormancy release.The results showed that the StNF-YA8 gene was consistently up-regulated in expression during dormancy release in tubers.The StNF-YA8 gene was further cloned(GenBank No.XM_015309982.1),and its ORF was 591 bp,encoding 196 amino acids.The subcellular localization vector of StNF-YA8 protein was constructed,and the results showed that StNF-YA8 protein was localized in the nucleus and cell membrane.Tissue-specific analysis revealed that the StNF-YA8 gene was differentially expressed between different tissues.The results of bioinformatics analysis showed that the potato StNF-YA8 protein contained a conserved CBFB domain,and had the highest evolutionary relationship with wild potato(S.commersonii);StNF-YA8 protein closely interacted with StNF-YB3/5/10/14/17 and StNF-YC1/4/5/8/9 proteins;there were cis-acting elements involved in light response,circadian rhythm control and hormone response in the promoter sequence of this gene.The results can provide a basis for the molecular mechanism analysis of potato StNF-YA8 gene involved in regulating tuber dormancy release.关键词
马铃薯/休眠/表达分析/核因子YA8(StNF-YA8)/生物信息学分析Key words
Potato/Dormancy/Expression analysis/Nuclear factor YA8(StNF-YA8)/Bioinformatics analysis分类
农业科技引用本文复制引用
王霄,魏涵,李世贵,王凯彤,赵亮,张宁,司怀军..马铃薯休眠解除相关基因StNF-YA8的表达分析[J].农业生物技术学报,2024,32(1):50-59,10.基金项目
国家自然科学基金(31960444) （31960444）
甘肃省科技重大专项(GZGG-2021-6) （GZGG-2021-6）
科技部对发展中国家科技援助项目(KY201901015) （KY201901015）
