农业生物技术学报2024，Vol.32Issue(1)：80-90,11.DOI:10.3969/j.issn.1674-7968.2024.01.008
芜菁种质资源根肿病抗性鉴定及组织显微观察
Identification of Resistance to Clubroot and Histological Microscopic Observation of Turnip(Brassica rapa ssp.rapifera)Germplasm
摘要
Abstract
Clubroot is one of the main diseases of cruciferous crops.Screening the clubroot resistant(CR)materials of turnip(Brassica rapa ssp.rapifera),clarifying the distribution of CR genes,and analyzing the disease-resistance mechanism in turnip can provide a theoretical and practical basis for the breeding of turnip CR varieties.In this study,the resistance of 25 turnip materials to clubroot was identified by inoculating Plasraodiophora brassicae at the artificial seedling stage,the resistance genes were detected by molecular markers,and the infection process of Plasraodiophora brassicae and pathological changes of root were observed by Phloxine-B staining and paraffin section techniques.As a results,4 materials(immune and highly resistant),among which WJ-9,WJ-21,and WJ-22 exhibited immunity,while WJ-14 exhibited high resistance.In addition,WJ-10,WJ-13,and WJ-18 exhibit disease tolerance;and 18 susceptible(susceptible and highly susceptible)materials were identified.The total number of CR genes in the tested materials was in descending order:Crr3＞CRb＞Crr1a=CrrA5＞CRc＞Crr1＞CRa.WJ-22 was the material with the most resistance genes,containing a total of 6 resistance genes.There were differences in the infection process and pathological changes of Plasmodiophora brassicae in resistant and susceptible turnip roots.Compared with the susceptible material,the root hairs were infected later,the infection amount was less,and the cortex was not infected in the resistant materials.The root cells of the susceptible material would swell after Plasmodiophora brassicae invade,and the structure would be disordered.Swollen cells squeezed and destroyed root canals.The cell structure of the disease-resistant material was completed.In this study,4 turnip resources with resistance to clubroot were identified,the CR genes carried by them were clarified,and the infection process and tissue structure differences between resistant and susceptible materials were explored.It provides a scientific basis for cultivating varieties with durable resistance to clubroot.关键词
芜菁/根肿病/抗性鉴定/分子检测/显微观察Key words
Turnip/Clubroot/Resistance evaluation/Molecular detection/Microscopic observation分类
农业科技引用本文复制引用
雷霆,刘晓乐,周晓青,杨昕蕾,惠麦侠..芜菁种质资源根肿病抗性鉴定及组织显微观察[J].农业生物技术学报,2024,32(1):80-90,11.基金项目
国家现代农业产业技术体系(CARS-23-G22) （CARS-23-G22）
陕西省农业专项项目(商业化育种2021-26) （商业化育种2021-26）
陕西省重点研发计划项目(2020NY-061) （2020NY-061）
太白省级农业科技园区重点建设项目(2022TBNYKJ-2) （2022TBNYKJ-2）
