森林草莓SAUR基因家族的鉴定与表达分析OACSTPCD

SAUR(small auxin up RNA)is a gene family that can respond quickly to auxin indole-3-acetic acid(IAA)treatment in the early stage,but how the gene family responds to auxin treatment in strawberries(Fragaria×ananassa)has not been reported.Therefore,this study used bioinformatics tools to analyze the evolutionary relationship,protein physical and chemical properties,gene structure and promoter elements of forest strawberry(F.vesca)SAUR gene family,as well as the changes of expression patterns of respond IAA.The results showed that 67 SAUR family members were identified in the forest strawberry genome.The amino acid number of these members varied from 83 to 286 aa.Except FvSAUR9,FvSAUR15,FvSAUR22,FvSAUR44,FvSAUR45 were hydrophobic,and the other members were hydrophilic.Subcellular localization prediction analysis showed that the expression of members of the gene family was mainly located in nucleus,mitochondria,chloroplast and cytoplasm.The results of phylogenetic tree showed that the SAUR gene members of Arabidopsis thaliana,forest strawberry and strawberry were divided into 10 subgroups.Among them,subgroup 5 had the highest number of members in F.vesca,with a total of 20 members.Additionally,subgroup 4 and subgroup 8 did not have any members.A total of 11 different types of elements were identified in the promoter sequences of forest strawberry SAUR gene members,included 5 types related to environmental stress and 6 types related to hormones,with the highest number of light responsive elements.The expression pattern of IAA in response of SAUR family members of forest strawberry was analyzed by qRT-PCR.The results showed that the expression levels of most members were significantly down-regulated after 2 h of IAA treatment(P＜0.05),while 12 members were up-regulated after 12 h,and all members of the SAUR gene family members were up-regulated after 24 h of treatment.In addition,the results of collinearity analysis showed that there were 6 gene pairs in the SAUR gene family of forest strawberries with collinearity.These results provide a reference for strawberry SAUR gene family members in growth and development and hormone response,especially in response to IAA treatment.