1-苯基2-硫脲通过p53调控自噬活性来保护斑马鱼神经丘毛细胞OACSTPCD

To illustrate the relationship between autophagy and hair cell survival,we labeled the neuro-mast in zebrafish with Lyso Tracker to track the autophagy in vivo.We found that 1-phenyl 2-thiourea(PTU)treatment induced the Lyso Tracker signaling enhancement in hair cells specifically,and also re-sulted in an increase in the number of hair cells and the expression of tp53 gene.To clarify the survival and protection mechanisms of hair cells,we constructed the tp53 mutant zebrafish by CRISPR/Cas9 technology.The tp53 mutation did not affect the number of hair cells in neuromast.However,the tp53 mutation reduced the enhancement of autophagy in hair cell induced by PTU,and lowered the number of hair cells increased by PTU treatment.Our evidence shows that PTU can promote autophagy in hair cells of lateral line neuromast in zebrafish by upregulating the expression of tp53 gene,thereby promo-ting the survival of hair cells,laying an important foundation for hair cell protection.