乳业科学与技术2023，Vol.46Issue(4)：10-15,6.DOI:10.7506/rykxyjs1671-5187-20230719-035
蛋白酶处理对牛乳乳清蛋白线性过敏表位的影响
Effect of Protease Treatment on Linear Allergenic Epitopes of Bovine Whey Proteins
王宗洲 1崔春兰 1李琴 1田其英 1张丽芳 1邵虎 1梁肖娜 2岳喜庆3
作者信息
- 1. 江苏食品药品职业技术学院食品学院,江苏 淮安 223003
- 2. 淮阴师范学院生命科学学院,江苏 淮安 223300
- 3. 沈阳农业大学食品学院,辽宁 沈阳 110866
- 折叠
摘要
Abstract
In this study,the bioinformatics software DNAStar and Geneious and multiple reaction monitoring mass spectrometry(MRM-MS)were used to predict the linear epitope parameters,secondary structure content and epitope regions of bovine whey proteins.Meanwhile,relative quantification of linear allergenic epitopes was carried out.The results indicated that α-lactalbumin(α-LA)and β-lactoglobulin(β-LG)had 7 and 10 linear epitope regions,respectively.The secondary structure of α-LA and β-LG mainly consisted of α-helix,followed by β-turn,β-sheet and random curling,indicating that the structure of α-LA and β-LG made them more likely to become epitopes.Alkaline,protamex and flavourzyme treatment effectively reduced the linear allergenic epitopes of α-LA and β-LG by 50.0%-80.0%,with this effect being more pronounced for β-LG.The relative quantitation results showed that the content of 95.0%of linear epitope peptides was significantly reduced.关键词
蛋白酶/酶解/乳清蛋白/线性表位Key words
protease/enzymatic hydrolysis/whey protein/linear epitope分类
轻工纺织引用本文复制引用
王宗洲,崔春兰,李琴,田其英,张丽芳,邵虎,梁肖娜,岳喜庆..蛋白酶处理对牛乳乳清蛋白线性过敏表位的影响[J].乳业科学与技术,2023,46(4):10-15,6.
