乳业科学与技术2023，Vol.46Issue(4)：22-27,6.DOI:10.7506/rykxyjs1671-5187-20230801-037
不同标准菌株对原料乳大肠菌群检验用培养基验收差异分析
Analysis of the Difference in the Quality Acceptance of Various Culture Media for the Detection of Coliform Bacteria in Raw Milk Using Different Standard Strains
摘要
Abstract
In order to ensure the quality of raw milk and enrich quality control strains for the acceptance evaluation of culture media for the detection of coliform bacteria in raw milk,three brands of violet red bile agar(VRBA),violet red bile agar with 4-methylumbelliferyl-β-D-glucuronidehydrate(VRBA-MUG),lauryl tryptose broth(LST)and brilliant green lactose bile broth(BGLB)were selected and evaluated for their physical indicators such as appearance,pH and moisture content as well as microbial indicators such as growth rate and selectivity using the methods specified in China's national standard for quality requirements for culture media and reagents in food microbiological examination(GB 4789.28-2013).Besides,using Citrobacter freundii ATCC 43864 as a reference strain,seven strains of C.freundii were selected for acceptance evaluation of the four media.The results showed that the color of all three brands of dry powdered media was normal.Their moisture content was 5%-6%,and their pH was in line with the national standard.The growth rate of the seven tested strains of C.freundii was greater than 0.7 on the three brands of VRBA and VRBA-MUG,while only CMCC 48098 and CICC 22910 achieved turbidity of 2 on the three brands of LST and BGLB,and produced gas in the catheter;the same results were obtained with ATCC 43864.In summary,the three brands of VRBA,VRBA-MUG,LST and BGLB all comply with GB 4789.28-2013,and CMCC 48098 and CICC 22910 can be recommended as supplements to quality control strains for the acceptance evaluation of the four media.关键词
原料乳/食品检验用培养基/大肠菌群/质控菌株/质量控制Key words
raw milk/media for food testing/coliforms/quality control strains/quality control分类
轻工纺织引用本文复制引用
刘帅,许苗苗,陈怡文,张捷,王慧,李献,刘雨蒙,崔生辉,张雅伦..不同标准菌株对原料乳大肠菌群检验用培养基验收差异分析[J].乳业科学与技术,2023,46(4):22-27,6.基金项目
河北省重点研发计划项目(22375503D) （22375503D）
河北省市场监督管理局科研计划项目(2022ZD15) （2022ZD15）
