乳业科学与技术2023，Vol.46Issue(4)：48-54,7.DOI:10.7506/rykxyjs1671-5187-20230517-024
超高效液相色谱串联三重四极杆质谱法测定婴幼儿配方乳粉中磺胺类和喹诺酮类药物残留
Determination of Sulfonamide and Quinolone Residues in Infant Formula Milk Powder by Ultra-Performance Liquid Chromatography Tandem Triple Quadrupole Mass Spectrometry
摘要
Abstract
An analytical method was established for the determination of the residues of 11 sulfonamides and five quinolones in infant formula milk powder using ultra-performance liquid chromatography tandem triple quadrupole mass spectrometry(UPLC-MS/MS).Acidified acetonitrile was used for sample extraction and the extract was purified and concentrated by solid phase extraction(SPE)using a PRiME hydrophilic lipophilic balance(HLB)column,then separated on an XBridge BEH C18 column,detected in the positive ion mode using an electrospray ionization source,and quantified by the calibration curve method with matrix matching.The calibration curves of all analytes showed a good linear relationship in the concentration range of 2-100 ng/mL with correlation coefficients greater than 0.995 0.The limit of detection(LOD)and the limit of quantification(LOQ)were 0.1-0.2 and 0.3-0.5 μg/kg,respectively.Recoveries for spiked samples were between 84.8%and 108.0%,with relative standard deviations(RSDs)between 0.36%and 5.41%.The proposed method is simple,efficient,reliable and sensitive.关键词
婴幼儿配方乳粉/磺胺/喹诺酮/超高效液相色谱串联三重四极杆质谱法/PRiME HLB固相萃取柱Key words
infant formula milk powder/sulfonamides/quinolones/ultra-performance liquid chromatography tandem mass spectrometry/PRiME HLB solid phase extraction column分类
化学化工引用本文复制引用
孙磊,张春林,何亮娜,范力欣,张旭,马俊美..超高效液相色谱串联三重四极杆质谱法测定婴幼儿配方乳粉中磺胺类和喹诺酮类药物残留[J].乳业科学与技术,2023,46(4):48-54,7.基金项目
河北省高层次人才资助项目(A202101055) （A202101055）
河北省市场监督管理局科研计划项目(2021ZD14) （2021ZD14）
