食品工业科技2024，Vol.45Issue(1)：155-161,7.DOI:10.13386/j.issn1002-0306.2023030055
临沂和周口地区中高温大曲细菌群落结构与基因功能差异性研究
Research on the Differences in Bacteria Community Structure and Gene Function of Medium-High Temperature Daqu in Linyi and Zhoukou Areas
摘要
Abstract
In order to clarify the differences in the community structure and gene function of medium-high temperature Daqu bacteria in Linyi,Shandong Province and Zhoukou,Henan Province.MiSeq high-throughput sequencing technology was used to analyze the bacterial community structure of medium-high temperature Daqu in the two regions,and the potential functions of bacterial taxa were analyzed based on the clusters of orthologous groups of proteins(COG).The result of α diversity showed that there were no significant differences in richness and diversity(P>0.05).Compared with Daqu in Zhoukou area,at the phylum level,the average relative abundance of Actinobacteria and Bacteroidetes in Daqu in Linyi area were significantly lower(P<0.05).At the genus level,the average relative abundance of Acinetobacter was significantly higher(P<0.05),and the average relative abundance of Geobacter and Pantoea were significantly lower(P<0.05).The result of linear discriminant analysis effect size(LEfSe)showed that Geobacter was a biomarker in Zhoukou area Daqu,while the biomarker in Linyi area Daqu was not reflected at the genus level.The result of β diversity showed that there were obvious differences as a whole.The result showed that Daqu in Linyi area was significantly higher than Daqu in Zhoukou area in transcription,amino acid and nucleoside transport and metabolic functions by phylogenetic investigation of communities by reconstruction of unobserved states(PICRUSt)(P<0.05).Thus,there are obvious differences in bacterial community structure and gene function expression in the two regions.关键词
地域/中高温大曲/细菌多样性/高通量测序/基因功能Key words
region/medium-high temperature Daqu/bacterial diversity/high-throughput sequencing/gene function分类
轻工纺织引用本文复制引用
马梦月,郭壮,李学思,管桂坤,刘宇,李富强,王玉荣..临沂和周口地区中高温大曲细菌群落结构与基因功能差异性研究[J].食品工业科技,2024,45(1):155-161,7.基金项目
湖北文理学院教师科研能力培育基金"科技创新团队"(2020kypytd009). （2020kypytd009）
