食品工业科技2024，Vol.45Issue(1)：185-191,7.DOI:10.13386/j.issn1002-0306.2023030025
银柴胡多糖超声辅助提取工艺优化及抗氧化活性分析
Optimization of Ultrasonic-assisted Extraction Process and Analysis of Antioxidant Activity of Polysaccharide from Stellariae Radix
摘要
Abstract
Objective:The aim of this study was to enhance the polysaccharide yield from Stellariae Radix and evaluate its in vitro antioxidant activity.Methods:Ultrasonic-assisted extraction method was employed to extract polysaccharides from Stellariae Radix,the single-factor experiment was combined with the Box-Behnken response surface to optimize the parameters of the extraction process.Additionally,Sevag method was utilized for the removal of protein to obtain crude polysaccharide of Stellariae Radix,which was further analyzed for its antioxidant activity.Results:The optimized conditions for extracting polysaccharides from Stellariae Radix were an ultrasonic temperature of 50℃,a time of 3.20 h,and an extraction frequency of 2 times,resulting in a yield of 28.24%±0.10%.The content of polysaccharide was 59.13%.Notably,the crude polysaccharide demonstrated antioxidant activity with IC50 values of 5.47,2.40 and 1.44 mg/mL for DPPH,OH,and ABTS+ radical scavenging activities,respectively.Conclusion:The optimized extraction process of polysaccharides from Stellariae Radix is feasible and can provide a theoretical foundation for the development and utilization of Stellariae Radix.关键词
银柴胡/多糖/提取/Box-Behnken响应面法/抗氧化活性Key words
Stellariae Radix/polysaccharides/extraction/Box-Behnken response surface/antioxidant activity分类
医药卫生引用本文复制引用
王红,彭励,宋乐,冯璐,李振凯,李彦青,高跳..银柴胡多糖超声辅助提取工艺优化及抗氧化活性分析[J].食品工业科技,2024,45(1):185-191,7.基金项目
宁夏重点研发计划项目(2021BEG02042) （2021BEG02042）
宁夏区自然基金(2021AAC03103). （2021AAC03103）
