食品工业科技2024，Vol.45Issue(1)：192-198,7.DOI:10.13386/j.issn1002-0306.2023030027
紫菜多糖硫酸化修饰及生物活性分析
Sulfated Modification and Bioactivity Analysis of Polysaccharide from Porphyra
摘要
Abstract
The polysaccharides were obtained by microwave-assisted enzymatic extraction from Porphyra,and then modified by sulphate method.Single factor and orthogonal experiments were used to optimize the sulfated modification process of polysaccharides,which were identified by Fourier transform infrared spectroscopy(FT-IR).The antioxidant and hypoglycemic activity changes of the polysaccharides modified by sulphate and chlorosulfonic acid-pyridine method were also studied.Results showed that the optimal technology parameters were the ratio of solid to liquid 1:80 g/mL,the mass ratio of Porphyra polysaccharide to ammonium sulfate 10:9 g/g,reaction time 33 min,and the maximum degree of substitution was 2.94.FT-IR showed that the characteristic absorption peaks of sulfate radical group appeared near 801 and 1123 cm-1,and the possible substitution position of sulfate radical group was C-6.The sulfate modification could significantly improve the scavenging ability of DPPH,O2-and OH free radicals and the inhibitory effect on α-glucosidase activity of the polysaccharides(P<0.05).The antioxidant and hypoglycemic activity of the polysaccharides modified by sulphate method were both higher than that by chlorosulfonic acid-pyridine method(P<0.05),indicating that sulphate method was applicable to modify the Porphyra polysaccharide.This study provides a theoretical basis for the further development of polysaccharides from Porphyra as a functional food.关键词
紫菜/多糖/硫酸化/抗氧化活性/降血糖活性Key words
Porphyra/polysaccharide/sulfated/antioxidant activity/hypoglycemic activity分类
轻工纺织引用本文复制引用
李瑶,熊欣琪,徐畅,冯学珍,冯书珍..紫菜多糖硫酸化修饰及生物活性分析[J].食品工业科技,2024,45(1):192-198,7.基金项目
国家自然科学基金项目(32001208) （32001208）
中央引导地方科技发展资金项目资助(桂科AD20159063) （桂科AD20159063）
博士资金项目(校科博 17Z05) （校科博 17Z05）
广西高校中青年教师科研基础能力提升项目(2019KY0366). （2019KY0366）
