食品工业科技2024，Vol.45Issue(1)：277-283,7.DOI:10.13386/j.issn1002-0306.2023030044
超高效液相色谱-串联质谱法检测卤肉中4种β-受体激动剂残留
Determination of 4 Kinds of β-Agonists Residues in Braised Meat by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
摘要
Abstract
An ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS)method was developed for the determination of four β-agonists(terbutaline,clenbuterol,ractopamine,salbutamol)in braised meat.Samples were hydrolyzed by β-glucuronidase and cleaned up by an SLS solid phase extraction column.The separation was performed on a Thermo Hypersil Gold C18 column with a gradient elution of 0.1%formic acid water and acetonitrile as mobile phases,ESI+ was used for multiple response monitoring(MRM)and quantitative analysis by internal standard method.The linear relationship of the four β-agonists was good in the concentration range of 0.5 μg/L to 9.5 μg/L,and the correlation coefficient(r)was greater than 0.9988.The limit of detection(LOD)was 0.1 μg/kg,and the limit of quantitation(LOQ)was 0.3 μg/kg.The recoveries were 87.9%～113.7%and RSDs were 1.48%～9.32%at three spiked levels(1,5 and 9 μg/kg).In a total of 162 batches of braised meat samples,one sample of braised pig's trotter was found to contain 1.51 μg/kg of clenbuterol and 3.65 μg/kg of ractopamine.Additionally,another sample of braised lamb was found to contain 11.5 μg/kg of clenbuterol.The method is rapid and accurate,and can be used for qualitative and quantitative determination of fourβ-agonists(terbutaline,clenbuterol,ractopamine,salbutamol)in braised meat.关键词
超高效液相色谱串联质谱(UPLC-MS)/卤肉/β-受体激动剂/SLS固相萃取柱/同位素内标Key words
ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS)/braised meat/β-agonists/SLS solid phase extraction column/isotope internal standard分类
化学化工引用本文复制引用
孟春杨,吴玉田,彭蕾,钟雪,邹璐,刘文政,周贻兵..超高效液相色谱-串联质谱法检测卤肉中4种β-受体激动剂残留[J].食品工业科技,2024,45(1):277-283,7.基金项目
贵州省卫生健康委科学技术基金项目(gzwkj2022-268) （gzwkj2022-268）
贵州省科学(青年)技术基金项目(2021-E1-2 青). （青年）
