孙璇 1李萍 2孙静 1郑嘉妮 1余丹阳 1张艳美 2陈朋1
作者信息
- 1. 仙乐健康科技股份有限公司,广东汕头 515000
- 2. 汕头大学医学院药理教研室,广东汕头 515041
- 折叠
摘要
Abstract
This study aims to investigate the protective effects of the extract of Sophora japonica L.(Sophora extract)and vitamin C(VC)on UV-induced damage in HaCaT cells.HaCaT cells were cultured in vitro and divided into different groups:Control group(without UV irradiation or treatment with Sophora extract,VC,or their combination),UV radiation group,125 μg/mL Sophora extract group(with UV irradiation and treatment with Sophora extract),125 μg/mL vitamin C group(with UV irradiation and treatment with VC),and 125 μg/mL combination group(with UV irradiation,Sophora extract and VC at a mass ratio of 1:1).The inhibitory effects of Sophora extract,VC,and their combination on reactive oxygen species(ROS)production and mitochondrial ROS in UV-irradiated HaCaT cells were measured using fluorescence staining and enzyme-linked immunosorbent assay.Immunofluorescence staining and enzyme-linked immunosorbent assay were also employed to evaluate the expression levels of cyclobutane pyrimidine dimers(CPDs)and the inflammatory cytokine IL-6 in UV-irradiated HaCaT cells treated with Sophora extract,VC,or their combination.The results showed that the total ROS inhibition rates of Sophora extract,VC,and the combination in UVA-treated HaCaT cells were 38.23%±9.23%,27.20%±6.87%,and 64.13%±4.08%,respectively.The combination significantly enhanced the total ROS inhibition rate compared to the individual treatments(P<0.01).The inhibition rates of mitochondrial ROS(mtROS)by Sophora extract,VC,and the combination were 50.29%±4.92%,38.81%±8.66%,and 84.74%±3.68%,respectively.The combination significantly increased the mtROS inhibition rate compared to the individual treatments(P<0.01).After UVB irradiation,the CPDs content in HaCaT cells increased,and Sophora extract,VC,and their combination significantly reduced CPDs formation(P<0.01),with the combination showing a more pronounced effect compared to the Sophora extract group(P<0.05)and the VC group(P<0.01)alone.Furthermore,Sophora extract and the combination significantly reduced the secretion of the inflammatory cytokine IL-6 in HaCaT cells after UVA irradiation(P<0.01,P<0.05),indicating an anti-inflammatory capability.The results demonstrated that the combination of Sophora extract and Vitamin C could alleviate oxidative stress damage and inflammatory responses caused by UV radiation and provided experimental support for future in vivostudies and the development and utilization of health-promoting Sophoraextract and VC combinations.关键词
槐提取物/维生素C/HaCaT细胞/抗氧化活性/炎症因子Key words
Sophora japonica L./vitamin C/HaCaT cells/antioxidant activity/inflammatory cytokines分类
轻工纺织
