食品工业科技2024，Vol.45Issue(1)：310-317,8.DOI:10.13386/j.issn1002-0306.2023030050
姜黄联合山楂对C57肥胖小鼠的减肥作用及机制研究
Study on the Weight Loss Effect and Mechanism of Turmeric Combined with Hawthorn on C57 Obese Mice
摘要
Abstract
Objective:To evaluate the weight loss effect of turmeric combined with hawthorn and explore its weight loss mechanism.Methods:Thirty-two C57BL/6J mice were randomly divided into four groups:normal group,normal intervention group,high-fat group and high-fat intervention group,8 mice in each group.The mice were gavaged with the corresponding drugs regularly every day,and the changes of adiposity,leanness,food intake and water consumption were detected regularly every week.The random blood glucose of the mice was measured at the same time every two weeks,after 12 weeks,the mice were removed from the cervical spine and the specimens were collected.The contents of total cholesterol(TC),triglyceride(TG),high density lipoprotein cholesterol(HDL-C),low density lipoprotein cholesterol(LDL-C),alanine aminotransferase(ALT),aspartate aminotransferase(AST)were measured in serum.The morphological changes of fat and liver tissue were observed by HE staining of epididymal fat and liver.The relative mRNA expressions of cholesterol 7α-hydroxylase(CYP7A1),fatty acid synthase(FAS),Liver X Receptorα(LXRα),Sterol Regulatory Element Binding Protein 2(SREBP2),Sterol Regulatory Element Binding Protein(SREBP1C),Steary-coenzyme A dehydro-synthase-1(SCD1)in liver were measured by real time polymerase chain reaction(RT-PCR).Results:Compared with the high-fat group,the body mass,fat percentage,TG,TC,LDL-C of mice in the high-fat intervention group were significantly decreased,lean meat percentage and HDL-C were significantly increased(P<0.05),and there was no significant difference in food intake and water intake.It could inhibit adipocyte hypertrophy and reduce fat deposition.And their liver cell vacuolation and inflammatory infiltration were improved.The mRNA expression level of CYP7A1 in liver was significantly increased(P<0.05).The mRNA expression levels of LXRα,SREBP1C,SCD and FAS were extremely significantly decreased(P<0.01).There was no significant change in the expression level of SREBP2 mRNA.Conclusion:Turmeric-hawthorn has good weight loss effects,and its weight loss mechanism is related to the regulation of mRNA expression levels in related genes.关键词
姜黄/山楂/C57小鼠/减肥作用/机制Key words
turmeric/hawthorn/C57 mice/weight loss effect/mechanism分类
轻工纺织引用本文复制引用
李强,李亚婷,徐华健,郝宗围,陈鹏浩,司雄元,汪雪雁..姜黄联合山楂对C57肥胖小鼠的减肥作用及机制研究[J].食品工业科技,2024,45(1):310-317,8.基金项目
国家"重大新药创制"科技重大专项资助项目(2018ZX09J18102-001-003) （2018ZX09J18102-001-003）
安徽省教育厅质量工程教学研究项目(2019KFKC040). （2019KFKC040）
