食品与发酵工业2023，Vol.49Issue(23)：1-8,8.DOI:10.13995/j.cnki.11-1802/ts.035579
重组大肠杆菌补救途径合成2'-岩藻糖基乳糖的分子克隆和产物合成分析
Construction of engineered Escherichia coli for 2'-fucosyllactose production via salvage pathway
摘要
Abstract
2'-Fucosyllactose(2'-FL)is one of the most abundant oligosaccharides found in human milk,which delivering many health benefits to the neonate.It is now available in some commercial infant formulas with great market potentials.In this study,salvage pathway was constructed by comparing α-1,2-fucosyltransferases from different sources,and choosing the co-expression of fucT2 from He-licobacter pylori and fkp.Then by over-expressing the substrate transporter LacY and product transporter Tpyb or SetA,the extracellular concentration of 2'-FL was greatly increased.Comparing the production capacity of BL21(DE3),C41(DE3),and JM109(DE3),the 2'-FL synthetic strain J2-V8 was obtained.Finally,through the optimization of fermentation conditions,the extracellular concentration of 2'-FL was 2.67 g/L in shaker for 48 h,which was 5 times higher than that of the initial strain,and the conversion rate was 0.82 mol/mol L-fucose,which provided a reference for the biological production technology of 2'-FL.关键词
人乳寡糖/2'-岩藻糖基乳糖/补救途径/微生物细胞工厂/生物合成Key words
human milk oligosaccharides/2'-fucosyllactose/salvage pathway/microbial cell factory/bio-synthesis引用本文复制引用
李娜,徐铮,韩子钰,徐虹,李古月,夏洪志,牛堃..重组大肠杆菌补救途径合成2'-岩藻糖基乳糖的分子克隆和产物合成分析[J].食品与发酵工业,2023,49(23):1-8,8.基金项目
国家重点研发计划项目(2018YFA0902200) （2018YFA0902200）
江苏省农业科技自主创新资金资助项目(CX(21)3133) （CX(21）
江苏省科技计划(社会发展-面上项目)(BE2021678) （社会发展-面上项目）
