食品与发酵工业2023，Vol.49Issue(23)：56-63,8.DOI:10.13995/j.cnki.11-1802/ts.035434
谷氨酸棒杆菌中L-半胱氨酸外排蛋白的鉴定及应用
Identification and application of L-cysteine exporter from Corynebacterium glutamicum
摘要
Abstract
As an important sulfur-containing amino acid,L-cysteine is widely used in food,medicine,cosmetics,and other indus-tries.Efficient transporters play an important role in the construction of cell factories,they not only reduce feedback inhibition and cyto-toxicity,but also improve cell production capacity.In this study,a novel L-cysteine exporter,Cg1298-Cgl299,was discovered in Coryne-bacterium glutamicum by transcriptome analysis.When 7.5 mmol/L L-cysteine was added,the cell growth of cg1298-cg1299 deletion strain was 24.2％lower than that of the control strain,while the complemented strain recovered its resistance to L-cysteine.In addition,the cysteine efflux capacity of Cg1298-Cg1299 was further examined by a dipeptide Cys-Tyr addition assay,and the results revealed that the efflux capacity of the cg1298-cg1299 deletion strain for L-cysteine decreased by 26.3％compared to the control strain.Finally,the production of L-cysteine increased by 17.1％by overexpression of cg1298-cg1299.In summary,our results showed that Cg1298-Cg1299 was a novel L-cysteine exporter in C.glutamicum,which significantly improved the tolerance of C.glutamicum to L-cysteine and the pro-duction of L-cysteine,providing an effective target for constructing efficient cell factory to produce L-cysteine in the future.关键词
L-半胱氨酸/谷氨酸棒杆菌/外排蛋白/转录组分析/Cg1298-Cg1299Key words
L-cysteine/Corynebacterium glutamicum/exporter/transcriptome analysis/Cg1298-Cg1299引用本文复制引用
乔晋芳,杜焕敏,刘川,祁玉婷,吴硕,徐宁,戴玉杰,邵丽,刘君..谷氨酸棒杆菌中L-半胱氨酸外排蛋白的鉴定及应用[J].食品与发酵工业,2023,49(23):56-63,8.基金项目
国家重点研发计划项目(2021YFC2100700) （2021YFC2100700）
