食品与发酵工业2023，Vol.49Issue(23)：64-69,6.DOI:10.13995/j.cnki.11-1802/ts.035035
双歧杆菌源亚油酸异构酶的原核表达及功能分析
Prokaryotic expression and functional analysis of linoleic acid isomerase from Bifidobacterium breve
摘要
Abstract
Bifidobacterium breve isomerase(BBI),the linoleic acid isomerase from Bifidobacterium breve CCFM683,is the only known single enzyme from lactic acid bacteria that catalyzes the formation of linoleic acid into cis9,trans11-CLA.Using pET-28a(+)as expression vector to construct recombinant vectors pET-28a(+)-bbi-His and pET-28a(+)-His-bbi with His-tag located at C-terminal and N-terminal,which was heterologous expressed in Escherichia coli BL21(DE3),E.coli BL21(DE3)pLysS and E.coli Rosetta(DE3)re-spectively.The optimum inductive condition for BBI expression is E.coli Rosetta/pET-28a(+)-bbi-His under the induction of 1.0 mmol/L IPTG for 8 h.BBI prefers α-linolenic acid to linoleic acid and y-linolenic acid when it catalyzed fatty acid.关键词
大肠杆菌/亚油酸异构酶/共轭脂肪酸/条件优化/底物偏好性Key words
Escherichia coli/linoleic acid isomerase/conjugated fatty acids/condition optimization/substrate selectivity引用本文复制引用
嵇熠彬,梅永超,杨波,陈海琴..双歧杆菌源亚油酸异构酶的原核表达及功能分析[J].食品与发酵工业,2023,49(23):64-69,6.基金项目
国家自然科学基金项目(32072186) （32072186）
