食品与发酵工业2023，Vol.49Issue(23)：76-83,8.DOI:10.13995/j.cnki.11-1802/ts.035024
1株非酿酒酵母2-苯乙醇合成规律及相关特性的初步研究
A preliminary study on the synthesis and related characteristics of 2-phenylethanol from a non-Saccharomyces cerevisiae strain
摘要
Abstract
As an important quorum-sensing molecule in Saccharomyces cerevisiae and alcohols,which have important contributions to wine aroma,the synthesis and regulatory mechanism of 2-phenylethanol(2-PE)have been studied in S.cerevisiae,but less in non-Sac-charomyces cerevisiae.It is difficult to understand the physiological and metabolic interactions between non-S.cerevisiae and S.cerevisiae induced by 2-PE in a mixed fermentation system and its impact on wine flavor.In this study,we screened a non-S.cerevisiae strain Mp-57,with dimorphic transformation using a restricted nitrogen source medium,and the 2-PE in the culture medium of Mp-57 strain was identified and detected by gas chromatography.The synthesis of 2-PE was also investigated.First,we found that the synthesis of 2-PE by Mp-57 strain depended on cell density.When the cell density reached 107 CFU/mL,2-PE was detected in the culture medium,and the threshold cell density was the same as that of S.cerevisiae.Second,the synthesis rate of 2-PE showed a bell-shaped curve;that is,the synthesis rate increased with the extension of culture time at the beginning,reached the maximum value at the end of the logarithmic stage,and then rapidly decreased.As a result,the 2-PE content in the supernatant increased continuously from the beginning to the end of the quiescent period and remained at a high level.In contrast to previous studies,non-S.cerevisiae Mp-57 showed stronger 2-PE syn-thesis ability,which was significantly higher than that of S.cerevisiae Ds.Finally,the effects of 2-PE on the fermentation properties of Mp-57 and Ds were investigated,and it was found that 2-PE was similar to the signal molecule,which affected the growth of Mp-57 and Ds in a dose-dependent manner.It promoted yeast growth at a concentration of 50 μmol/L and inhibited yeast growth at a concentration of 500 µmol/L.Moreover,2-PE at 50 µmol/L improved the tolerance of Mp-57 and Ds to ethanol and significantly promoted the formation of biofilm of strain Mp-57.The results provide a theoretical basis and experimental strains for studying the interaction mechanism between non-S.cerevisiae and S.cerevisiae in a mixed fermentation system.关键词
限氮/非酿酒酵母/2-苯乙醇/群体感应/生物被膜/乙醇耐受性/二型态转换Key words
nitrogen restriction/non-Saccharomyces cerevisiae/2-phenylethanol/quorum sensing/biofilm/ethanol tolerance/dimorphic transformation引用本文复制引用
王琮杰,钱云开,阎贺静,崔宗岩,石文琪,罗莉莎,肖艳霞,单超,葛超..1株非酿酒酵母2-苯乙醇合成规律及相关特性的初步研究[J].食品与发酵工业,2023,49(23):76-83,8.基金项目
河北科技师范学院科学研究基金项目(2020YB009) （2020YB009）
河北省重点研发计划项目(19222810D) （19222810D）
