食品与发酵工业2023，Vol.49Issue(23)：111-118,中插1,9.DOI:10.13995/j.cnki.11-1802/ts.035228
萝卜泡菜母水中乳酸菌分离鉴定与发酵特性比较
Isolation,identification,and fermentation characteristics of lactic acid bacteria from the radish pickles brines
摘要
Abstract
The dominant lactic acid bacteria(LAB)strains were isolated and identified from the traditional fermented Sichuan radish pickle brines,and their fermentation characteristics were examined.Isolated strains were identified by physiological and biochemical ex-periments,as well as 16S rDNA sequencing.The fermentation characteristics of the strains were evaluated including their growth perform-ance,tolerance to salt and nitrite and nitrite degradation capability,compared to strain Weissella paramesenteroides.Gas chromatography-ion mobility spectroscopy(GC-IMS)was used to detect and analyze the volatile flavor substances to evaluate the flavor-producing capabili-ty of the strains.Isolated LAB strains L2,L3,L7,L15,and L24 were identified respectively as Lactobacillus buchneri,Lactobacillus bre-vis,Pediococcus acidilactici,Lactiplantibacillus plantarun and Lacticaseibacillus rhamnosus.Strains LI 5 and L24 grew faster than others.Strains L7,L15,and L24 showed stronger acid production capability.Strains L15 and L24 showed better tolerance to NaCl.Strain L7 showed the worst tolerance to sodium nitrite.All five strains showed capability to degrade nitrite by more than 90％.The volatile sub-stances produced by the five strains were different.L3 produced the highest content of ketones.L15 produced the highest relative content of alcohols.L24 produced the highest relative content of esters.In summary,all five lactic acid bacteria have the potential to ferment pickles and can be used as functional microbial resources for pickle production.关键词
乳酸菌/分离鉴定/耐受性/发酵特性/挥发性成分Key words
lactic acid bacteria/isolation and identification/tolerance/fermentation characteristics/volatile components引用本文复制引用
胡此海,杨絮,郭全友,李保国,郑尧,黄海潮,范逸文..萝卜泡菜母水中乳酸菌分离鉴定与发酵特性比较[J].食品与发酵工业,2023,49(23):111-118,中插1,9.基金项目
中国水产科学研究院基本科研业务费资助项目(2023TD68) （2023TD68）
