食品与发酵工业2023，Vol.49Issue(23)：173-180,8.DOI:10.13995/j.cnki.11-1802/ts.033915
叶绿素与膜分离乳蛋白相互作用及复合物性质研究
Interaction between chlorophyll and milk protein from membrane separation and the properties of their complexes
摘要
Abstract
Chlorophyll is a kind of natural pigment with multiple bioactivities but low stability,which is distributed in higher plants extensively.In this study,the binding constants between chlorophyll and micellar casein,whey protein,and skim milk protein were ana-lyzed by using fluorescence spectroscopy,and the structures of their spray-dried complexes were characterized by Fourier transform infra-red spectroscopy,and scanning electron microscope as well.The solubility,thermal stability,and chromaticity of the complexes were evaluated,as well as the release properties of chlorophyll from the complexes during simulated gastrointestinal digestion.Results showed that the binding constants between chlorophyll and milk proteins were all higher than 106 L/mol.After combining with chlorophyll,the morphology of most spray-dried whey protein changed from a raisin-like shape to a hemispherical one with a hollow structure,but those of micellar casein and skim milk protein were not altered.The binding of chlorophyll improved the solubility of three kinds of milk proteins in the pH range of 4.5-8.In addition,loaded milk protein enhanced the color stability of chlorophyll during 30-day storage.After 24 h simulated digestion,the cumulative release rate of free chlorophyll was the lowest and the cumulative release rate of the micellar casein-chlorophyll complex was the highest.Thus,the complexation with milk proteins enhanced the stability of chlorophyll in simulated gastroin-testinal digestion fluids.Results would provide the basic references for the application of chlorophyll in functional foods,as well as milk proteins.关键词
叶绿素/酪蛋白胶束/乳清蛋白/脱脂乳/结合常数/溶解性/色度Key words
chlorophyll/micellar casein/whey protein/skim milk/binding constant/solubility/chromaticity引用本文复制引用
苏小红,刘成力,杨敏,季伟,魏玉梅..叶绿素与膜分离乳蛋白相互作用及复合物性质研究[J].食品与发酵工业,2023,49(23):173-180,8.基金项目
兰州市科技计划项目(2021-1-147) （2021-1-147）
国家自然科学基金项目(32060549) （32060549）
甘肃省自然科学基金项目(21JR1RA207) （21JR1RA207）
