食品与发酵工业2023，Vol.49Issue(24)：1-7,7.DOI:10.13995/j.cnki.11-1802/ts.034996
基于代谢组学分析理性强化三羧酸循环提高L-丝氨酸产量
Enhancing tricarboxylic acid cycle based on comparative metabolomics analysis to increase L-serine production
摘要
Abstract
L-serine is widely used in medicine,food and cosmetic industry,however,the industrial production of L-serine by micro-bial fermentation has not yet been realized.In previous studies,we found that there had a significant difference in L-serine titer by Coryne-bacterium glutamicum A using sucrose and glucose.In this paper,comparative metabolomics analysis method was used to study the differ-ences of intracellular metabolites of C.glutamicum A in different carbon sources,revealed the key metabolites of L-serine synthesis,and then investigated the influence of the addition of key metabolites on L-serine production.Through GC-MS,principal component analysis,and partial least squares discriminant analysis,15 kinds of intracellular metabolites were found to be closely related to L-serine synthesis,including glucose,glucose 6-phosphate,3-phosphoglycerate,pyruvate,fructose 6-phosphate,alanine,succinic acid,fumaric acid,citric acid,malic acid,phenylalanine,tryptophan,proline,glycine and methionine,among which fumaric acid,succinic acid,α-ketoglutaric acid,malic acid,and citric acid in the tricarboxylic acid cycle had great influence on the synthesis of L-serine.The fermentation results showed that the addition of 2.5 g/L fumaric acid had the significant inhibition on the cell growth,and the biomass(OD562)decreased by 33.9％.Although fumaric acid was added to inhibit the cell growth,it was significantly beneficial to L-serine production.When 2 g/L fu-maric acid was added,L-serine titer increased by 29.8％.These results showed that the optimal medium based on comparative metabolo-mics analysis could improve the L-serine production.关键词
谷氨酸棒杆菌/L-丝氨酸/代谢组学/理性优化/微生物发酵Key words
Corynebacterium glutamicum/L-serine/metabolomics/rational optimization/microbial fermentation引用本文复制引用
陈剑,张晓梅,史劲松,许正宏..基于代谢组学分析理性强化三羧酸循环提高L-丝氨酸产量[J].食品与发酵工业,2023,49(24):1-7,7.基金项目
国家自然科学基金项目(32171470) （32171470）
