食品与发酵工业2023，Vol.49Issue(24)：44-51,8.DOI:10.13995/j.cnki.11-1802/ts.034890
堇叶碎米荠富硒多肽对乙醇诱导THLE-2肝细胞损伤的保护作用
Protective effect of selenium-enriched peptide from Cardamine violifolia on ethanol-induced THLE-2 hepatocyte injury
摘要
Abstract
Human hepatocytes were treated with a selenium-enriched peptide from Cardamine violifolia(SPE)to study whether SPE could interfere with ethanol-induced hepatocyte injury.An oxidative stress model was constructed via the alcohol-induced injury of THLE-2 hepatocytes.After treatment with SPE at different concentrations of organic selenium,the cell injury and oxidative stress related indica-tors were measured to evaluate the cell protection and antioxidant capacity.The results showed that the concentration of ethanol of 1 000 mmol/L and the action time of ethanol of 12 h led to significant damage of THLE-2 cells(P＜0.05).SPE treatment significantly in-creased the cell viability(P＜0.05),which reached 78.02％at selenium concentration of 12 μmol/L.Compared with the injury group,the contents of aspartate aminotransferase,alanine aminotransferase,and lactate dehydrogenase in the supernatant of SPE treatment group was significantly reduced(P＜0.05).Moreover,after SPE treatment,the content of reactive oxygen species in THLE-2 cells was decreased,and the levels of superoxide dismutase,glutathione peroxidase,catalase,and glutathione were significantly increased(P＜0.05),with the highest values of 34.68 U/mg,974.75 nmol/g,1.76 U/mg,and 55.58 μmol/g,respectively;among them,glutathi-one peroxidase was highly expressed,at about 5.6 times that of the injury group,probably because SPE provided selenium ions for gluta-thione peroxidase synthesis.In addition,the apoptosis rate was decreased,and the mitochondrial membrane potential was increased in the SPE treatment group.Above all,the selenium-enriched polypeptide of Cardamine violifolia alleviated alcoholic liver injury by regulating oxidative stress related indicators.关键词
堇叶碎米荠富硒多肽/酒精性肝损伤/THLE-2/抗氧化/有机硒Key words
selenium-enriched peptide of Cardamine violifolia/alcoholic liver damage/THLE-2/antioxidant/organoselenium引用本文复制引用
凌荣瑞,丛欣,陈尚卫,朱松..堇叶碎米荠富硒多肽对乙醇诱导THLE-2肝细胞损伤的保护作用[J].食品与发酵工业,2023,49(24):44-51,8.基金项目
国家自然科学基金项目(32172197) （32172197）
